生脉分散片中人参皂苷Rgl、Re总量和五味子醇甲的纯化工艺研究  

Study on Technical Processes for Purification of Ginsenosides Rgl,Re and Schizandrin from Shengmai Dispersible Tablets

作　　者：蒋亚超[1] 朱志军[1] 张楠楠[1] 邓浩[1] 高松[1] 陈耀升[2] 

机构地区：[1]河南中医药大学,河南郑州450046 [2]郑州市第二人民医院,河南郑州450008

出　　处：《中医学报》2016年第12期1950-1953,共4页Acta Chinese Medicine

基　　金：河南中医药大学研究生创新基金(2012YCX001)

摘　　要：目的:确定生脉分散片中主要有效成分人参皂苷Rgl、Re总量和五味子醇甲的纯化工艺。方法:以生脉分散片中人参皂苷Rgl、Re总量和五味子醇甲含量为指标,比较不同型号大孔吸附树脂和不同工艺条件对生脉分散片有效成分的分离纯化能力。结果:采用D-101大孔吸附树脂对人参皂苷Rg1、Re总量和五味子醇甲的吸附容量分别为4.918 5 mg·g^(-1)和11.177 6 mg·g^(-1);解吸率分别为56.05%,33.87%,为综合最佳吸附树脂。优选最佳纯化工艺为:上药液质量浓度0.16 kg·L^(-1),上样体积为9 BV,上样速率2 BV·h^(-1);6 BV水平衡,体积分数70%乙醇6 BV洗脱,速率均为4 BV·h^(-1)。结论:本工艺条件操作简单,人参皂苷Rgl、Re总量和五味子醇甲纯度均提高20倍以上,回收率分别达到59.18%,67.78%,适用于生脉分散片的纯化工艺。Objective： To optimize the purification technology of ginsenosides Rg1,Re and Schizandrin in Shengmai Dispersible Tablets. Methods： The purification conditions were examined by using the amount of total ginsenosides Rgl,Re and Schizandrin as main indicators,and the purification conditions of the active components from Shengmai Dispersible Tablets were optimized by different types of macroporous resin and different technological conditions. Results： The optimal purification process was as follows： using d^-101 macroporous resin,the adsorption capacity and desorption rate of ginsenosides Rgl,Re and Schizandrin were 4. 918 5mg·g- 1,11. 177 6 mg·g^-1and 6. 05%,56. 05% respectively; added 9BV（ 9 times the volume） of 0. 16 g·m L^-1sample with the rate of 2 BV·h^-1（ 2 times the volume per hour） and 6BV water and 70% ethanol as elution with a rate of 4 BV·h^-1. Conclusion： The optimal purification process of Shengmai Dispersible Tablets is simple stable. The purity of ginsenosides Rgl,Re and Schizandrin increased 20 times than before. The rate of recovery were 59. 18%,67. 78% respectively by purification. The optimal technical processes for purification are practical for Shengmai Dispersible Tablets.

关 键 词：生脉分散片 大孔树脂 纯化 人参皂苷RGL 人参皂苷RE 五味子醇甲 中药 

分 类 号：R284.2[医药卫生—中药学]