基于药效学和指标成分优选鼻鼽颗粒醇沉工艺  

Optimization of alcohol precipitation technology of Biqiu granules based on index components and pharmacodynamics

作　　者：张艳军[1,2] 刘莉莉[1,2] 吴云[1,2] 胡军华[1,2] 常秀娟[1,2] 萧伟[1,2] 晁恩祥[3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药提取精制新技术重点研究室,江苏连云港222001 [3]中日友好医院,北京100029

出　　处：《中国中药杂志》2016年第24期4598-4604,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：通过考察对血清组胺,Ig E,IL-4,IFN及TNF-α的影响,评价鼻鼽颗粒醇沉工艺的合理性。以咖啡酸和迷迭香酸的含量为综合指标,采用Plackett-Burman设计筛选影响鼻鼽颗粒水提组醇沉工艺的显著性影响因素;在此基础上,通过Box-Behenken设计对显著性因素进一步优化,确定最佳醇沉工艺。确定鼻鼽颗粒水提组的最佳醇沉工艺条件为:浓缩液相对密度1.15(65℃)、醇浓度70%、静止时间12 h。药效学评价结合Plackett-Burman和Box-Behnken设计实验优化确定的醇沉工艺稳定可行,预测性好,为鼻鼽颗粒的产业化生产提供了可靠依据。To evaluate the rationality of alcohol precipitation technology of Biqiu granule by investigating its effect on serum hista- mine, IgE, IL-4, IFN and TNF-α. The contents of cafferic acid and rosmarinic acid were used as the evaluation indexes, and some factors affecting index were firstly evaluated by Plackett-Burman design; then alcohol precipitation technology was further optimized by Box-Behnken design to determine the optimal alcohol precipitation conditions. The best alcohol precipitation conditions were as follows： the relative density of herb liquor was 1.15 （65 ℃ ） ; the concentration of alcohol was 70%, and standing time was 12 hours. Optimal alcohol precipitation technology of Biqiu granules determined by pharmacodynamic screening, Plackett-Burman and Box-Behnken design tests, was stable and feasible with good predictability, providing reliable basis for the industrialization production of Biqiu granules.

关 键 词：鼻鼽颗粒 药效学 PLACKETT-BURMAN设计 BOX-BEHNKEN设计 醇沉工艺 

分 类 号：R283.6[医药卫生—中药学]