剑叶凤尾蕨化学成分及其细胞毒活性研究  

Chemical constituents from Pteris multifida and cytotoxic activity

作　　者：张艳[1] 石玉生[2] 胡文忠[2] 宋丽艳[1] 陈效忠[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]大连民族大学生命科学学院生物技术与资源利用国家民委-教育部共建重点实验室,辽宁大连116600

出　　处：《中国中药杂志》2016年第24期4610-4614,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31471923;31172009)

摘　　要：采用95%乙醇提取药材,提取液回收至无醇味后依次过聚酰胺树脂、MCI树脂、中压制备液相色谱和制备型高效液相色谱,从剑叶凤尾蕨全草中分离得到8个二萜类化合物和2个倍半萜类化合物。质谱（ESI-MS）数据和核磁共振（NMR）数据与文献数据对比鉴定分离得到的化合物分别为ent-3β-hydroxy-kaur-16-en-19-al（1）,4-epi-kaurenic acid（2）,mitrekaurenone（3）,7β,16α,17-trihydroxy-ent-kauran-19-oic acid（4）,crotonkinin E（5）,crotonkinin F（6）,pterisolic acid A（7）,pterisolic acid C（8）,（2R）-pterosin P（9）和dehydropterosin B（10）。化合物1~6首次从凤尾蕨属中分离得到,化合物7~10首次从剑叶凤尾蕨中获得。细胞毒活性测试表明化合物5~8具有一定的抑制人结肠癌细胞HCT-116、肝癌细胞Hep G2和人胃癌细胞BGC-823的活性。The materials were extracted by 95% ethanol, and the extracting solution was isolated by kinds of chromatographic col- umns including polyamide, MCI, preparative MPLC, and preparative HPLC. Eight diterpenes and two sesquiterpenes were isolated from the plant. On analysis of ESI-MS and NMR spectroscopic data, the structures were established as ent-3fl-hydroxy-kaur-16-en-19- al （ 1 ）, 4-epi-kaurenic acid （2）, mitrekaurenone （3）, 7/3,16a, 17-trihydroxy-ent-kauran-19-oic acid （ 4 ）, crotonkinin E （ 5 ）, cro- tonkinin F （6）, pterisolic acid A （7）, pterisolic acid C （8）, （2R）-pterosin P （9）, and dehydropterosin B （10）. Compounds 1-6 were obtained from Pteris for the first time, and compounds 7-10 were obtained from P. ensiformis for the first time. Compounds 5-8 showed moderate activity against HCT-116, HepG2 and BGC-823 cell lines, separately.

关 键 词：剑叶凤尾蕨 二萜 倍半萜 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]