荆芥挥发油对内毒素中毒模型小鼠的保护作用  

Protective effects of Schizonepeta volatile oil on endotoxin poisoning induced by lipopolysaccharide in mice

作　　者：温桃群 桑文涛 徐锋[1] 王凤[1] 曾南[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中国中药杂志》2016年第24期4642-4647,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473399);中药药理四川省青年科技创新团队项目(2014TD0007)

摘　　要：观察荆芥挥发油对内毒素(LPS)中毒模型小鼠的保护作用,并采用GC-MS对荆芥挥发油进行化学成分测定。雄性C57BL/6J小鼠按体重分层随机分为空白对照组、模型对照组、地塞米松组(5 mg·kg-1)、荆芥挥发油0.226 g·kg-1及0.452g·kg-1剂量组。除地塞米松组实验当日腹腔注射给药一次外,其余各组小鼠连续灌胃给药5 d,1次/d。各组末次给药30min后,除空白组小鼠外,其余各组小鼠腹腔注射LPS(15 mg·kg-1)制备内毒素中毒模型。造模12 h后,小鼠取血分离血清,采用ELISA及液相蛋白芯片技术测定炎症因子IL-18,IL-1β,IL-5,TNF-α,单核细胞趋化蛋白-1(MCP-1),巨噬细胞炎性蛋白(MIP-1β),巨噬细胞集落刺激因子(M-CSF)及粒细胞-巨噬细胞集落刺激因子(GM-CSF)的水平;剖取肺脏、脾脏、胸腺称重,计算脏器指数;取小鼠全血进行白细胞(WBC)、血小板(PLT)计数;同时进行各组小鼠肺组织病理组织学检查。GC-MS检测结果显示薄荷酮、胡薄荷酮的相对质量分数分别为46.67%,33.92%,二者质量分数占总挥发油的80.59%。荆芥挥发油预防给药,0.452,0.226 g·kg-1剂量均能显著降低模型小鼠血清IL-1β,IL-5,TNF-α,MCP-1,MIP-1β,M-CSF水平(P<0.01或P<0.05),荆芥挥发油0.452 g·kg-1剂量亦降低血清IL-18,GM-CSF水平(P<0.01或P<0.05);荆芥挥发油0.226 g·kg-1剂量能使肺组织内嗜中性粒细胞浸润减少,显示出良好抗炎效应。但荆芥挥发油预防给药,对模型小鼠白细胞计数的升高、血小板计数的减少及脾脏指数、肺指数的升高、胸腺指数的降低无明显干预作用。结果表明,荆芥挥发油预防给药对内毒素(LPS)中毒模型小鼠有一定保护作用,作用发挥主要与抑制各类炎性细胞因子的释放,减轻炎症反应有关。In order to study the protective effects of Schizonepeta volatile oil （Sto）on endotoxin poisoning mice, and the relatively content of each chemical osubstance in Schizonepeta volatile oil was measured using GC-MS. The mare C57BL/6J mice were randomly divided into five groups including the normal group, model group, dexamethasone group （5 mg · kg^- 1 ） , and Sto （0. 226 and 0. 452 g · kg^-1, respectively） groups. The dexamethasone group was given the drugs once time by intraperitoneal injection on the 5th day, while the other mice were given drugs by oral administration once a day for 5 days. Then, the normal group was injected with the saline and the other groups were injected LPS （15 mg · kg^-1） after 30 minutes of the last administration. After LPS injection twelve hours, the blood, serum, and lung tissue of mice were collected. The IL-18, IL-1β, IL-5, TNF-α, MCP-1, MIP-1β, M-CSF, and GM-CSF were measured in serum by ELISA and Luminex Magpix. The white cell （WBC） and platelet （PLT） in blood were counted and lung,spleen, and thymus index were calculated. The lung histopathology was performed at the same time. The GC-MS results showed that the relative content of menthone and pulegone are 46. 67% and 33.92% , respectively. The Sto （0. 452 and 0. 226 g · kg^-1 , respec- tively） reduced the levels of IL-1β, IL-5, TNF-α, MCP-1, MIP-1β, and M-CSF in serum （P 〈 0. 01 or P 〈 0.05）. The 0. 452 g ·kg^- 1 Sto also reduced the levels of IL-18 and GM-CSF in the serum （P 〈 0. 01 or P 〈 0. 05 ）. And the 0. 226 g · kg^- 1 Sto showed good anti-inflammatory effects by reducing neutrophil infiltration in the lung tissue. But the Sto had no effect on the increasing of WBC, spleen and lung index as well as decreasing of PLT and thymus index. The results showed that Sto has a protective effect in LPS-in- duced exdotoxin poisoning mice, its mechanism is related to inhibit the release of varies of inflammatory cytokines and reduce the in- flammation reaction.

关 键 词：荆芥挥发油 内毒素中毒 保护作用 小鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]