离子色谱法测定水果和蔬菜中2,4-二氯苯氧乙酸的含量  

IC Determination of 2,4-Dichlorophenoxyacetic Acid in Fruits and Vegetables

作　　者：赵士权[1] 查河霞[1] 赵好[1] 

机构地区：[1]南京市疾病预防控制中心,南京210003

出　　处：《理化检验（化学分册）》2016年第12期1435-1438,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用离子色谱法测定水果和蔬菜中2，4-二氯苯氧乙酸的含量。水果或蔬菜样品10．0g经0．2mol·L^-1硫酸溶液10．0mL提取后，用二氯甲烷10．0mL萃取，二氯甲烷层再用35mmol·L^-1氢氧化钾溶液5．00mL反萃取，取水相，经AG-19保护柱及AS-19阴离子分离柱分离，以35mmol·L^-1氢氧化钾溶液淋洗色谱柱，所得淋出液采用抑制电导检测器检测。2，4-二氯苯氧乙酸的质量浓度在5．0mg·L^-1以内与其峰面积呈线性关系，检出限（3S／N）为0．05mg·L^-1。加标回收率在93．6％-105％之间，测定值的相对标准偏差（n=6）在2．1％-5．5％之间。IC was applied to the determination of 2, 4-dichlorophenoxyacetic acid in fruits and vegetable. Sample of fruit or vegetable （10. 0g） was extracted with 10. 0 mL of 0. 2 mol·L^-1 H2SO4 solution, and then extracted with 10. 0 mL of dichlorornethane. The organic layer was reverse extracted with 5.00 rnL of 35 mmol·L^-1 KOH solution. The aqueous phase was separated on AG-19 protective column and AS19 anion separation column, and the analyte was eluted from the column with 35 mmol·L^-1 KOH solution. Inhibitorty conductivity detection was adopted in the determination. Linear relationship between values of peak area and mass concentration of 2,4-dichlorophenoxyacetic acid was kept in the range within 5. 0 mg·L^-1, with detection limit （3S/N） of 0. 05 mg·L^-1. Values of recovery found by standard addition method were in the range of 93.6%-105%, and values of RSD＇s （n=6） found were in the range of 2.1%-5.5%.

关 键 词：离子色谱法 水果 蔬菜 2 4-二氯苯氧乙酸 

分 类 号：O657.7[理学—分析化学]