果胶乙酰酯酶基因AtPae7参与拟南芥对大豆疫霉菌的非寄主抗病性  

AtPae7 Encodes a Pectin Acetyl Esterase and Involved in Non-host Resistance to Phytophthora sojae

作　　者：杨志远[1] 王钢[1] 曹华[1] 蓝星杰 尹军良[1] 单卫星[1,2] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2016年第11期1716-1722,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：现代农业产业技术体系(CARS10)~~

摘　　要：利用反向遗传学方法,鉴定拟南芥(Arabidopsis thaliana)对大豆疫霉菌(Phytophthora sojae)非寄主抗病性相关的基因。通过分子生物学和生物信息学方法,鉴定和分析T-DNA插入拟南芥突变体离体叶片对大豆疫霉菌的非寄主抗病性。结果表明:4个果胶乙酰酯酶基因AtPae7(pectin acetyl esterase 7)突变体株系中的3个对大豆疫霉菌的抗性减弱,表现为感病;qRT-PCR检测表明突变体中AtPae7的转录水平下降;生物信息学预测PAE7蛋白的N端有23个氨基酸信号肽序列,确定其为胞外分泌蛋白。说明果胶乙酰酯酶基因AtPae7参与拟南芥对大豆疫霉菌的非寄主抗病性。This study is aimed at identification and characterization of genes related in non-host resistance in T-DNA insertional Arabidopsis thaliana mutants to Phytophthora sojae based on reversed genetics.A.thaliana T-DNA insertional mutants were identified and characterized with their resistance to P.sojaeinfecting detached leaves by molecular biological and bioinformatics methods.Three of four pae7（pectin acetyl esterase 7）mutants presented a decrease of resistance to P.sojae and became susceptible.Transcriptional level of pae7 in its mutants declined based on detection by qRT-PCR.Bioinformatics analysis predicted that there were signal peptides of 23 amino acids at N-end of PAE7 protein,the results suggested that it is extracellular secreted protein.A.thaliana pae7 plays a role in non-host resistance of A.thalianato P.sojae.

关 键 词：卵菌 T-DNA插入突变体 非寄主互作 果胶乙酰酯酶 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]