羧甲基壳聚糖修饰的磁性纳米粒应用于分离生物碱  

Separation of alkaloids using superparamagnetic carboxymethyl chitosan nanoparticles

作　　者：洪小巧 张亚平[1] 赵焕焕[1] 侯健[1] 刘莹[1] 周采菊[1] 

机构地区：[1]济宁医学院药学院,日照276826

出　　处：《分析试验室》2016年第12期1389-1391,共3页Chinese Journal of Analysis Laboratory

基　　金：山东省卫生厅项目(2014WS0512);济宁医学院大学生创新训练计划项目(cx2015059);济宁医学院重点科研项目(JY2013KJ008)

摘　　要：制备羧甲基壳聚糖磁性纳米粒,并用于分离生物碱以研究其吸附分离能力。共沉淀法合成Fe3O4磁性纳米粒;壳聚糖先经羧甲基化,再通过碳二亚胺活化共价结合到Fe3O4纳米表面上。采用傅立叶变换红外光谱、透射电子显微镜和振动样品磁强计对磁性纳米材料进行结构表征,表明磁性纳米具有单一分散性,平均粒径14.4 nm。同时将制得的磁性纳米粒应用于分离生物碱,并对分离提取条件包括萃取液pH、萃取时间、洗脱液种类和浓度等进行了优化。在298～328 K间焓变化（ΔH）为-5.80 kJ/mol,吸附过程自发进行,显示其具有较好的吸附分离能力。The carboxymethyl chitosan magnetic nanoparticles （CMC@ MNPs） have been prepared, and their separation capacity for the alkaloid in Chinese herbal medicine was evaluated. The Fe3 04 magnetic nanoparticles （MNPs） were synthesized by coprecipitation method. Chitosan was first carboxymethylated and then covalently bound on the surface of Fe3O4 nanoparticles via carbodiimide activation. The characteristics of prepared CMC@ MNPs were determined by transmission electron microscopy （TEM）, vibrating sample magnetometer, and Fourier transform infrared spectroscopy, respectively. TEM micrographs showed that the chitosan -bound Fe3O4 nanoparticles were monodisperse and had a mean diameter of 14.4 nm. These functional nanomaterials were also applied for the separation of the alkaloid. The separation conditions, such as the pH in the extract, the extraction time, the kind and the concentration of the eluent were optimized. The adsorption process was exothermic in nature with an enthalpy change of - 5.80 kJ/mol at 298 K -328 K.

关 键 词：羧甲基壳聚糖 磁性纳米粒 吸附分离 生物碱 

分 类 号：O636.1[理学—高分子化学]