固相萃取结合超高效液相色谱-串联质谱法测定植物提取物中6种工业染料  

Determination of six industrial dyes in plant extracts using solid-phase extraction coupled with ultraperformance liquid chromatography-tandem mass spectrometry

作　　者：蔡屹[1] 何强 张亚莉[2,3] 孔祥虹 刘开 张璐 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳518045 [2]陕西出入境检验检疫局技术中心,西安710068 [3]陕西科技大学,西安710021

出　　处：《分析试验室》2016年第12期1402-1407,共6页Chinese Journal of Analysis Laboratory

基　　金：国家质检总局科技项目(2014IK120;2015IK216)资助

摘　　要：建立了植物提取物中的碱性橙21、碱性橙22、碱性嫩黄O、罗丹明B、碱性橙2、酸性橙2等6种工业染料的液相色谱-串联质谱分析方法。样品经乙腈提取,固相萃取柱净化,使用Waters Acquity^(TM) UPLC BEH C18柱作为分析柱,以多反应监测(MRM)方式在ESI(±)模式下进行检测,外标法定量。结果表明:6种工业染料含量在5.0~50.0 ng/m L范围内线性良好,相关系数(r^2)均大于0.99;在5,10,25μg/kg 3个添加水平下回收率为65.0%~122.4%,相对标准偏差(RSD)在1.8%~12%(n=6)之间。方适用于植物提取物中多种工业染料的同时测定。A liquid chromatography- tandem mass spectrometry （LC -MS/MS） method was developed for the determination of six industrial dyes in plant extracts, including alkaline orange 21, alkaline orange 22, Auramine （0）, Rhodamine B, basic orange 2 and Acid Orange 2. Samples were extracted by acetonitrile, purified by solid phase extraction column, and separated on a Waters AcquityTM UPLC BEH C18 column. The MS analysis was performed on electrospray ion source （ESI） in positive and negative ion mode, multiple reaction monitoring （MRM） mode was used for ion signal acquisition, and the external standard method was adopted for quantification. The results showed good linearity in the range of 5.0 - 50.0 ng/mL, with correlation coefficients （r2） higher than 0.99. At the spiked levels of 5, 10 and 25 μg/kg, the average recoveries were in the range of 65.0%- 122.4% with the relative standard deviations from 1.8% to 12% （n =6）. This method is simple and sensitive, and can be used for the simultaneous determination of multiple industrial dyes in plant extracts.

关 键 词：固相萃取 超高效液相色谱-串联质谱 工业染料 植物提取物 

分 类 号：TQ075[化学工程]