在线富集毛细管区带电泳分离测定氯胺酮与去甲氯胺酮  

On-line preconcentration and determination of ketamine and norketamine by capillary zone electrophoresis

作　　者：刘光辉[1] 张佳瑶[1] 文元波 任周阳[2] 曹秋娥[1] 

机构地区：[1]云南大学化学科学与工程学院,昆明650091 [2]云南警官学院,昆明650223

出　　处：《分析试验室》2016年第12期1424-1427,共4页Chinese Journal of Analysis Laboratory

基　　金：公安部重点研究计划项目(201301ZDYJ008);公安部科技强警基础工作专项项目(2013GABJC018)资助

摘　　要：采用场放大样品堆积进样技术与毛细管区带电泳相结合,在优化了进样与分离检测条件后,建立了一种检测氯胺酮和去甲氯胺酮的毛细管区带电泳方法。方法于进样前先在3447.38 Pa压力下进10 s的纯水,然后在同样压力下进样5s,再以含15%（V/V）乙腈的45 mmol/L Na2HPO4-KH2PO4缓冲溶液（p H 6.24）为运行电解质进行分离。在此条件下,氯胺酮和去甲氯胺酮可在6min内被快速检测,检测的线性范围分别为0.5-60.0μg/m L和0.25-25.0μg/m L。将该方法用于加标尿液和血液样品中这两种成分的分离分析,回收率在90%-110%之间。A method was developed for the rapid, simple and sensitive determination of ketamine and norketamine by combining the capillary zone electrophoresis with a field amplified sample accumulation technique. A buffer solution composed of 15% （V/V） acetonitrile and 45 mmol/L KH2PO4 -Na2HPO4（pH 6. 24） was used for the separation of the two compounds after water was injected by pressure 3447.38 Pa for 10 s followed by injection of sample for 5 s. Ketamine and norketamine can be separated and determined within 6 min with the linear range of 0.5 - 60.0 μg/mL and 0.25 - 25.0 μg/mL, respectively. The developed method was used for the determination of the two compounds in urine and blood samples with recovery between 90% and 110% .

关 键 词：毛细管区带电泳 场放大样品堆积进样技术 氯胺酮 去甲氯胺酮 

分 类 号：O657.8[理学—分析化学]