抗汉滩病毒鼠/人嵌合抗体工程细胞的驯化、抗体表达及鉴定  

Acclimation of CHO Cells Expressing Human-Mouse Chimeric Antibody Against Hantanvirus in Serum- Free Medium and Identification of the Antibody

作　　者：张晓晓[1,2] 冯伟[3] 张尧[3] 程林峰[1] 应旗康[1] 马瑞雪[1] 马铁军[1] 刘梓谕[1] 刘蓉蓉[1] 王芳[1] 张亮[1] 叶伟[1] 张芳琳[1] 徐志凯[1] 吴兴安[1] 

机构地区：[1]第四军医大学微生物学教研室,陕西西安710032 [2]培华学院医学院,陕西西安710125 [3]第四军医大学学员旅,陕西西安710032

出　　处：《生物技术通讯》2016年第6期783-786,798,共5页Letters in Biotechnology

基　　金：国家自然科学基金(31470890;31270978);陕西省科技统筹创新工程计划(2013KTCL03-06);军队重点课题(BWS13G024)

摘　　要：目的:通过对稳定表达抗汉滩病毒鼠/人嵌合抗体的CHO细胞进行驯化,检测其抗体的表达情况,并对所表达抗体的生物学活性进行初步鉴定。方法:将本课题组前期筛选得到的稳定高表达细胞株1-D9进行无血清驯化,使其适合悬浮培养后接种到摇瓶,通过调整不同的培养条件,每天检测细胞的活率和抗体表达量,并对表达的抗体进行生物学活性鉴定。结果:通过驯化得到了适合悬浮培养的CHO细胞株,其抗体表达量高,生产周期短;并优化了其悬浮培养条件,表达的抗体经鉴定其生物学活性稳定。结论:优化了抗汉滩病毒鼠/人嵌合抗体表达条件,为下一步工业化生产提供了实验依据。Objective： To obtain a modified CHO cell line which can stably express mouse/human chimeric anti-body for anti-hantaanvirus（HTNV） and accommodate to serum-free medium for large scale production. Methods：The modified CHO cell line 1-D9 were adapted to serum-free medium using a gradient reduction of serum man-ner, and were transformed to a suspension cell at a specific condition, which was also adjusted for keeping the an-tibody viability and productivity. Results： We optimized the method of serum-free adaption and the suspended 1-D9. The cell line had advantages over original cell line in both antibody production and growth rate. Conclusion：Our work successfully optimized the condition of chimeric anti-HTNV antibody expression and could be a profit-able trial for industrialization of antibody production.

关 键 词：汉滩病毒 悬浮培养 CHO细胞 工业化生产 嵌合抗体 

分 类 号：Q813.11[生物学—生物工程] R392.1[医药卫生—免疫学]