尼古丁影响小鼠胚胎发育的分子机制初探  

A Preliminary Study on the Molecular Mechanism of Nicotine Influence on Mouse Embryo Development

作　　者：杜靖雯 王佳佳[1] 李书仪 焦琳[1] 李睿英[1] 韩斅[1] 胡海龙[1] 刘柏岐 吴琼[1] 

机构地区：[1]哈尔滨工业大学生命科学与技术学院,黑龙江哈尔滨150080

出　　处：《生物技术通讯》2016年第6期794-798,共5页Letters in Biotechnology

基　　金：哈尔滨工业大学大学生创新创业训练计划(国家级)

摘　　要：目的:探讨不同剂量的尼古丁对小鼠胚胎组织器官发育的影响及其分子机制。方法:以妊娠期雌鼠为评价模型,通过皮下注射不同浓度的尼古丁,在E18.5时解剖获取胚胎并称重,同时采集各组织器官,应用HE染色观察相关组织的病理学变化,并采用qRT-PCR检测相关基因表达水平的变化。结果:与对照组相比,尼古丁对小鼠E18.5胚胎的脑、肺、肝脏、肾脏有明显损伤;qRT-PCR分析显示血管形成相关基因(VEGF-α)、神经生长相关基因(Egr-1)及细胞生长分化相关调控基因(c-FOS)有显著的表达差异,其中VEGF-α在心脏和肾脏中表达下调,Egr-1在肺和肾脏中表达上调,c-FOS在脑、心脏和肺中表达上调。结论:妊娠期母鼠注射尼古丁会对小鼠胚胎的脑、肺、肝脏、肾脏产生明显损伤,并导致VEGF-a、Egr-1及c-FOS基因表达异常。Objective： To investigate the effects of different dosage of nicotine injection during pregnancy on or-gan development of mouse embryos and to claim the possible molecular mechanism of the observed effects. Methods： Maternal rats during the perinatal period were subcutaneously injected with nicotine and the embryos were ob-tained and weighted at the day of 18.5. Furthermore, HE staining was used to observe the pathologic differencesin the embryo tissue and these changes were partly explained by gene expression change through qRT-PCR tech-nique. Results： Compared with the control group, nicotine triggered a remarkable damage in E18.5 embryo likebrain, lung, liver and kidney. Further, VEGF-α, Egr-1 and c-FOS showed a significant difference in gene expres-sion. VEGF-α was up-regulated in heart and kidney, while Egr-1 was down-regulated in lung and kidney and cFOS was down-regulated in brain, heart and lung. Conclusion： The nicotine injection during pregnancy will exertan effect on the viscera development of mouse embryos and cause the differential gene expression of three genes,VEGF-α, Egr-1 and c-FOS.

关 键 词：尼古丁 小鼠胚胎发育 VEGF-A EGR-1 C-FOS 

分 类 号：Q24[生物学—细胞生物学]