贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究  

Genetic Diversity of Microsatellite LEI0258 in MHC-B Region of Royal Chicken

作　　者：黄勋和[1] 张金枫 陈洁波[1] 叶伟庆 李威娜[1] 钟福生[1] 杜炳旺[2] 

机构地区：[1]嘉应学院生命科学学院,梅州514015 [2]广东海洋大学农学院,湛江524088

出　　处：《中国畜牧兽医》2016年第12期3300-3305,共6页China Animal Husbandry & Veterinary Medicine

基　　金：广东省自然科学基金项目(2014A030307018);嘉应学院"创新强校工程"项目(CQX019);嘉应学院重点科技计划项目(2015KJM03);广东省科技计划项目(2015A020208020;2016A030303068);国家科技基础条件平台-特种动物子平台建设子课题(201520)

摘　　要：为研究贵妃鸡的遗传多样性,试验应用主要组织相容性复合体 B 区域（MHC-B）复合微卫星位点 LEI0258测定了贵妃鸡的等位基因信息,结合已发表的序列,进行等位基因与核苷酸多态性、单倍型中介网络图分析。40份样品中共检测到9个等位基因,长度为193-297 bp,其中5个为贵妃鸡特有。观察杂合度（Ho）、期望杂合度（He ）和多态信息含量（P IC）分别为0.625、0.716和0.666。3个等位基因与6种已知 MHC 血清型相匹配。基于侧翼区变异信息的中介网络图将等位基因分为5个进化枝,贵妃鸡9个等位基因分布于 A 和 B 进化枝中,这2个进化枝同样存在于红原鸡中。研究结果表明贵妃鸡保持着中等偏上的遗传多样性水平,可能起源于红原鸡。The aim of this study was to evaluate the polymorphism and evolution in royal chicken using the tandem repeat microsatellite LEI0258 located within the chicken major histocompatibili-ty complex B region （MHC-B）.Genomic DNA was extracted from forty blood samples of royal chicken and used to be genotyped and sequenced.Combining the published sequences,the analysis of allele and nucleotide polymorphism and median-joining network were carried out.A total of nine alleles ranging from 1 93 to 297 bp in 40 samples were found,five of which was novel for royal chicken.The observed heterozygosity,expected heterozygosity and polymorphic information content were 0.625,0.71 6 and 0.666,respectively.Three alleles were corresponding to six availa-ble serotypes.Alleles were classified into five clusters,based on the SNPs and indels found within the sequences flanking the repeats.The alleles of royal chicken existed in clades A and B,both of which were also found in Red junglefowl.The results suggested that royal chicken had a middle or high level of genetic diversity in microsatellite LEI0258,and that might originate from Red jun-glefowl.

关 键 词：贵妃鸡 多态性 主要组织相容性复合体 微卫星位点LEI0258 进化 血清型 

分 类 号：S831.2[农业科学—畜牧学]