褐飞虱在不同水稻品系上繁殖能力及相关解毒酶系基因表达变化分析  

On the Fertility and Related Detoxification Enzyme Systems Gene Expression of Nilaparvata lugens Feeding on Different Rice Varieties

作　　者：俞姗姗[1] 刘雅[1] 杨萌萌[1] 沈祺达[1] 谢国强[1] 王世贵[1] 唐斌[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2016年第6期595-599,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31371996);杭州市科技局计划项目(20140432B01);浙江省大学生科技创新活动计划暨新苗人才计划项目(2015R423043);杭州师范大学"本科生创新能力提升工程"计划项目(CX2014088)

摘　　要：采用水稻敏感品系TN1和抗性品系中浙优、IR56饲养褐飞虱,研究褐飞虱产卵量等繁殖情况,测定解毒酶系中酯酶、细胞色素P450和谷胱甘肽转移酶(GST)及卵黄原蛋白(Vg)等基因在mRNA上的表达变化.结果显示褐飞虱在抗性水稻上产卵量减少,Vg2的表达量极显著下降,表明水稻抗性对褐飞虱的产卵量有抑制作用且Vg2调控产卵.酯酶和P450中的部分基因均在取食抗性水稻的褐飞虱体内表达量较高,存在极显著差异.This experiment adopts the TN1 sensitive rice varieties and other different levels of resistance rice cultivars , such as Zhongzheyou and IR56 ,to breed N .lugens ,and then use a part of those N .lugens to study N .lugens spawning and other breeding situations ,and determines the expression changes of esterase ,cytochrome P450 ,Glutathione transferase （GST） ,vitellogenin（Vg） on mRNA .The results show that the fecundity of N .lugens reduces on resistance rice ,and the expression of Vg2 decreases significantly ,which indicates resistance rice has inhibitory effect on N . lugens fecundity and Vg2 regulate the spawning .Part genes of esterase and P450 have high expression in the resistance rice N . lugens body , which have significant differences .

关 键 词：褐飞虱 抗性 解毒酶系 生化反应 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]