河北省健康人群肾综合征出血热疫苗免疫前后中和抗体水平研究  

Serum neutralizing antibody levels of healthy population after immunization with HFRS vaccine in Hebei Province

作　　者：李琦[1] 魏亚梅 韩旭[1] 韩占英[1] 蔡亚男[1] 张艳波[1] 许永刚[1] 齐顺祥[1] 

机构地区：[1]河北省疾病预防控制中心,河北石家庄050021

出　　处：《实用预防医学》2017年第1期40-42,共3页Practical Preventive Medicine

基　　金：河北省科技研究与发展计划项目(13277719D)

摘　　要：目的了解河北省健康人群肾综合征出血热病毒(HV)隐性感染情况,观察肾综合征出血热双价灭活疫苗的免疫效果。方法选择河北省滦南、北戴河、昌黎3个县为疫苗接种点,接种对象为16~60岁的健康人群(去除HFRS既往感染者及疫苗禁忌症者),用荧光抗体病毒中和试验(FAVN)的方法测定免疫前、免疫后中和抗体。结果疫苗免疫前人群血清HV中和抗体阳性率为2.92%,免疫后人群血清HV中和抗体阳性率(70.42%)比免疫前显著升高(P<0.05);免疫前HV中和抗体阳性率在各年龄段之间差异无统计学意义(P>0.05),免疫后各年龄组之间差异有统计学意义(P<0.05),且各年龄段及总人群免疫后抗体阳性率均显著高于免疫前(P<0.001);疫苗免疫后抗体滴度升高,各年龄组间抗体滴度差异无统计学意义(P>0.05)。结论肾综合征出血热双价灭活疫苗免疫效果显著,值得推广。Objective To investigate the inapparent infection of hantavirus （ HV ） in healthy population in Hebei Province, and to valuate the immune efficacy of a bivalent vaccine against hemorrhagic fever with renal syndrome （ HFRS ）. Methods Luannan County, Beidaihe County and Changli County were selected as the vaccination points, and the heahhy people aged 16-60 years except those suffered from HFRS previously and those with contraindications served as the inoculable objects. The titers of neutralizing antibody （ NA ） were detected by the fluorescent antibody virus neutralization （ FAVN ） assay before and after the immune inoculation. Results The positive rate of neutralizing antibody in the population after vaccination was significantly higher than that before vaccination （ 70.42% vs. 2.92%, P〈0.05 ）. If was not significantly different among different age groups before vaccination （P〉0.05）, but it was after vaccination （P〈0.05）. Moreover, the positive rates of neutralizing antibody after vaccination in each age group and the total population were all significantly higher than those before （ P〈0.001 ）. The titer of neutralizing antibody after vaccination was increased, but no statistically significant differences were found among different age groups （P〉0.05）. Conclusions The double-titer inactivated vaccine against HFRS has obvious immune efficacy. It is worth while spreading widely.

关 键 词：肾综合征出血热 双价肾综合征出血热灭活疫苗 中和抗体 防病效果 

分 类 号：R186[医药卫生—流行病学]