酸性染料比色法测定关黄柏中总生物碱的含量  

Determination of the Total Alkaloids of Cortex Phellodendri by Acid Dye Colorimetry

作　　者：蓝松[1] 尹艳 

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000 [2]宜春学院,江西宜春336000

出　　处：《广东化工》2016年第24期134-135,共2页Guangdong Chemical Industry

摘　　要：目的：评价关黄柏的质量以及其总生物碱的含量。方法：酸性染料比色法,在pH=5.2条件下,生物碱与酸性染料结合,即生成有吸收光谱明显红移的有色离子对,该离子对可以定量的被有机溶剂萃取,测定有机相中有色离子对特征波长处的吸光度,从而再进行关黄柏中总生物碱含量的测定,并进行回收率实验。结果：在415 nm处有最大吸收,酸性染料比色法的显色条件为pH=5.2的缓冲溶液8.0 mL,溴麝香草酚蓝溶液3.0 mL,氯仿萃取三次,每次4.0 mL,每次萃取振摇3分钟,静置0.5 h。盐酸小檗碱在55.2～166.5μg/mL范围内成线性关系,平均回收率为100.5%,RSD为2.0%（n=5）。结论：该方法用量少,灵敏度高、重复性好。具有一定的专属性和准确度。Subjective： Content quality evaluation of Cortex Phellodendri and its total alkaloids. Method： Acid dye colorimetry, In pH=5.2 conditions, the alkaloids combined with acid dyes, which generated the ion absorption Spectra clearly redshift, the ion-pair can be quantified by organic solvent extraction, determination of ferrous ion in organic solvents on the characteristic wavelength of absorbance, which then turn off the determination of content of Total alkaloids from cortex phellodendri, and recovery experiments. Results： At the maximum absorption at 415 nm, acid dye colorimetry color condition of 8.0 mL pH=5.2 buffer solution, bromothymol blue solution 3.0 mL, chloroform extracted three times, each 4.0 mL, each extraction shaking 3 minutes, allowed to stand for 0.5 h. Berberine hydrochloride as in 55.2～166.5 μg/mL range linear relationship, the average recovery was 100.5 %, RSD was 2.0 %（n=5）.

关 键 词：功劳木 柱色谱-紫外分光光度法 盐酸小檗碱 含量测定 

分 类 号：O65[理学—分析化学]