山胡椒根化学成分及其生物碱抑制肿瘤细胞增殖研究  

Chemical Constituents from the Roots of Lindera glauca and Their Antitumor Activity on Four Different Cancer Cell Lines

作　　者：刘婷[1] 李文艺[1] 刘小文[1] 齐成媚[1] 袁志辉[1] 

机构地区：[1]湖南科技学院化学与生物工程学院,湖南永州425199

出　　处：《中药材》2016年第8期1789-1792,共4页Journal of Chinese Medicinal Materials

基　　金：湖南省重点实验室开放基金项目(13113100310)

摘　　要：目的：对山胡椒根的化学成分进行研究,并探讨其分离得到的生物碱成分对4种肿瘤细胞株增殖抑制活性。方法：采用硅胶柱层析、RP-18和Sephadex LH-20等多种色谱分离方法并结合MS、NMR等波谱解析技术及与文献数据对比,鉴定山胡椒根中生物碱及萜类成分,对分离得到的生物碱进行抗肿瘤活性测试。结果：从山胡椒根共分离得到10个化合物,分别鉴定为：木兰箭毒碱（1）、N-甲基樟苍碱（2）、樟苍碱（3）、波尔定碱（4）、去甲异波尔定碱（5）、去甲异紫堇定碱（6）、对$烷-反式-3,8-二醇（7）、对$烷-顺式-3,8-二醇（8）、eudesm-4（15）-ene-7,11-diol（9）、4β,6β-dihydroxy-1α,5β（H）-guai-9-ene（10）。化合物2~4对4种肿瘤细胞HT-29、SGC-7901、SMMC-7721和A549增殖均具有抑制活性。结论：其中,化合物1~6为生物碱类成分,化合物1、9和10为首次从该植物中分离得到。化合物2具有强烈的抑制肿瘤细胞增殖活性,特别是抑制结肠癌细胞HT-29、胃癌细胞SGC-7901细胞增殖活性,在中、高浓度给药情况下,甚至优于同浓度的阳性对照药物依托泊苷VP-16。Objective： To study the chemical constitutes from the roots of Lindera glauca and the alkaloids influence on proliferation of HT-29,SGC-7901,SMMC-7721 and A549 cell lines. Methods： The constituents were isolated by column chromatography such as RP-18,Sephadex LH-20 and silica gel,and their structures were elucidated by spectroscopic data analysis and compared with literature data. The antitumor activity was determined by MTT assay. Results： Ten compounds had been isolated and identified as（-）-magnocurarine（ 1）,N-methyl-laurotetanine（ 2）,laurotetanine（ 3）,（ ＋）-boldine（ 4）,（-）-norisoboldine（ 5）,（ ＋）-norisocorydine（ 6）,pmethane-3,8-trans-diol（ 7）,p-methane-3,8-cis-diol（ 8）,eudesm-4（ 15）-ene-7,11-diol（ 9） and 4β,6β-dihydroxy-1α,5β（ H）-guai-9-ene（ 10）. Compounds 2 ~ 4 showed significant inhibitory activities against HT-29,SGC-7901,SMMC-7721 and A549 cells. Conclusion：Compound 1,9 and 10 are isolated from this plant for the first time. The IC50 value of compound 2 against HT-29 and SGC-7901 cell lines is even lower than VP-16.

关 键 词：山胡椒 生物碱 萜类 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]