FcγRIIb抑制B细胞内TLR4介导的CD40和CD80表达  

FcγRIIb inhibits TLR4-mediated CD40 and CD80 expression in B cells

作　　者：钱莉[1] 王少卿[1] 刘阳[1] 陈文艳[1] 龚卫娟[1] 田芳[1] 李国才[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001

出　　处：《扬州大学学报（农业与生命科学版）》2016年第3期15-19,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(81001308;81373130);江苏省自然科学基金资助项目(BK2010315)

摘　　要：利用磁珠法分选野生型和FcγRIIb缺陷小鼠脾脏CD19+B细胞,体外用LPS和/或FcγRIIb配体(IC)刺激24h后,流式细胞术(FCM)检测细胞表面共刺激分子的表达。用蛋白质印迹法检测IC刺激后B细胞内相关蛋白激酶的磷酸化情况。用Lyn抑制剂作用后,FCM检测LPS活化B细胞表面共刺激分子表达探讨B细胞内FcγRIIb对TLR4激动剂(LPS)诱导共刺激分子表达的影响。结果表明:IC抑制LPS活化B细胞表面CD40和CD80的表达,而在FcγRIIb缺陷小鼠B细胞内这一抑制作用消失。IC诱导B细胞内蛋白激酶Lyn的磷酸化,但不能诱导FcγRIIb缺陷小鼠B细胞内Lyn磷酸化。使用Lyn抑制剂后LPS活化B细胞表面CD40的表达上调。说明B细胞内FcγRIIb通过活化Lyn抑制TLR4激动剂诱导的CD40表达。Splenic B cells from wild type（WT）and FcγRIIb knockout mice were stimulated with LPS and/or FcγRIIb ligand（immune complex,IC）.After 24 h,cells were analyzed for costimulatory molecules expression by flow cytometry（FCM）.Western blot analysis was performed for phosphorylated protein kinases in lysates of splenic B cells stimulated with IC.FCM analysis was used for costimulatory molecules expression on splenic B cells pretreated with an inhibitor of Lyn,followed by LPS stimulation.Then,the effects of FcγRIIb on TLR4agonist（lipopolysaccharide,LPS）-induced costimulatory molecules expression in B cells were studied.showed that IC treatment inhibited CD40 and CD80expression on B cells stimulated with LPS.The inhibitory effect of IC on LPS-triggered CD40 and CD80expression was lost when B cells from FcγRIIb-/-mice were used.The phosphorylation of Lyn increased when WT B cells were stimulated with IC.However,the phosphorylation of Lyn showed a similar level when FcγRIIb-/-B cells were used.Furthermore,the inhibitor of Lyn enhanced the LPS-induced CD40 expression.FcγRIIb inhibits TLR4agonist-induced CD40 expression in B cells via activating Lyn.

关 键 词：FCΓRIIB TLR4 B细胞 

分 类 号：R392.1[医药卫生—免疫学]