南海新村湾海草沉积物细菌群落组成及分布  

The bacterial communities constitution and distribution in sediment of seagrass in Xincun Bay,South China Sea

作　　者：江玉凤[1,2] 凌娟[1] 董俊德[1,3] 张燕英[1,3] 

机构地区：[1]中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室,广州510301 [2]中国科学院大学,北京100049 [3]中国科学院海南热带海洋生物重点实验站,三亚572000

出　　处：《生物学杂志》2016年第6期38-42,共5页Journal of Biology

基　　金：国家自然科学基金(41276113;41276114;41406191;41676163);湖南省科技兴海专项(XH201415);广东省公益研究与能力建设专项(2015A020216016)

摘　　要：采用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）技术及产物序列分析方法,比较研究南海新村湾3个站位海草沉积物中细菌群落组成及分布。结果表明：根据DGGE图谱代表的遗传多样性显示春季二号和春季三号站位样品相似度最高为81.5%,秋季一号和秋季三号站位样品相似度最低为29.3%。春季和冬季细菌多样性指数较高,其Shannon指数范围分别为3.03~3.07和3.23~3.35。通过对16S r DNA V3高变区域42条条带克隆测序比对,结果显示：海草沉积物细菌类群主要分布在变形菌门（Proteobactetia）和厚壁菌门（Firmicutes）,其中变形菌门中Gammaproteobacteria含量最多,为新村湾海草沉积物中优势菌群,占总数的65.80%。蓝细菌（Cyanobacteria）普遍存在于所有沉积物样品中。细菌群落结构在不同季节、不同站位间具有一定差异。秋季二号站位和三号站位优势类群分别为嗜冷杆菌属和交替假单胞属,其含量分别为31.61%和19.41%。In order to compare the bacterial communities constitution and distribution in three sites of seagrass sediment in Xincun Bay, South China Sea, Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis （PCR-DGGE） analysis compounded with cloning and sequencing bands in DGGE profile was used. The results diverse bands of DGGE profile showed the most similarity of bac- terial communities was found between the second and the third site in spring with an coefficient of 81.5%, whereas the least was 29. 3% between the first and the third site in autumn. Shannon diversity indexes of the bacteria in spring and winter were higher than other seasons, which were of 3.03 - 3.07 and 3.23 - 3.25, respectively. Further, based on the 16S rDNA-V3 fragments from 42 indi- vidual DGGE bands and clone library sequence, the results showed most of the sequences belonged to Proteobactetia and Firmicutes. The most abundance Proteobactetia was Gammaproteobacteria with a percentage of 65.80%. Cyanobacteria were widely distributed in all samples. In addition, there were differences in bacterial communities among different seasons and sites. Psychrobacter and Pseudoalteromonas were advantage groups in the second and the third site during autumn, with a percentage of 31.61% and 19.41%, respectively.

关 键 词：海草沉积物 细菌群落组成 南海 新村湾 

分 类 号：Q938[生物学—微生物学]