手十二井穴放血对pMCAO大鼠脑组织血脑屏障通透性的影响  

The Effect of Hand Twelve Jing-Well Points Bloodletting on Blood-brain Barrier Permeability in PMCAO Rats

作　　者：陈宇岑 王振国 陈泽林[3] 

机构地区：[1]天津中医药大学,天津300193 [2]西安中医脑病医院,西安710068 [3]天津中医药大学针灸推拿学院,天津300193

出　　处：《上海针灸杂志》2016年第12期1466-1468,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：天津市科学技术委员会应用基础与前沿技术研究计划一般项目(14JCYBJC25800)

摘　　要：目的观察手十二井穴放血对永久大脑中动脉栓塞(Permanent Middle Cerebral Artery Occlusion,p MCAO)大鼠局灶性脑缺血损伤早期脑组织血脑屏障(Blood-brain Barrier,BBB)通透性的影响。方法选用雄性Wistar大鼠,随机分为假手术组、模型组和放血组,用Longa法建立大脑中动脉栓塞模型后在5 h、24 h、72 h 3个时间点尾静脉注射2%伊文思蓝(Evans Blue,EB),通过测量提取脑组织中EB吸光度来评估EB的渗出量,以观察BBB的通透性。结果模型组大鼠脑组织EB渗出量要显著高于假手术组(P<0.01),放血组大鼠的脑组织EB渗出量低于模型组(P<0.01)。结论手十二井穴刺络放血可以降低pMCAO大鼠血脑屏障通透性,其效果在缺血后24 h和72 h均极为显著。Objective To investigate the effect of hand twelve Jing-Well points bloodletting on blood-brain barrier （BBB） permeability in the early stage of focal cerebral ischemic injury in rats with permanent middle cerebral artery occlusion （pMCAO）. Method Male Wister rats were randomized into sham operation, model and bloodletting groups. A model of middle cerebral artery occlusion was made by Longa＇s method. 2%Evans blue （EB） was injected into the caudal vein at three time points：5, 24 and 72 hrs after model making. EB exudation amount was assessed by measurement of EB absorbance in brain tissue to observe BBB permeability. Result Cerebral EB exudation amount was significantly higher in the model group of rats than in the sham operation group （P〈0.01） and significantly lower in the bloodletting group than in the model group （P〈0.01）. Conclusion Hand twelve Jing-Well points bloodletting can reduce blood-brain barrier permeability in pMCAO rats. Its effect is highly significant at 24 and 72 hrs after ischemia.

关 键 词：穴 井 手十二井穴 放血疗法 永久大脑中动脉栓塞 大鼠 血脑屏障通透性 

分 类 号：R2-03[医药卫生—中医学]