Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil  

大豆异黄酮对饲喂氧化鱼油新生仔猪肠道抗氧化功能及细胞因子的影响（英文）

作　　者：Lin HUANG Xian-yong MA Zong-yong JIANG You-jun HU Chun-tian ZHENG Xue-fen YANG Li WANG Kai-guo GAO 

机构地区：[1]Institute of Animal Science, Guangdong Academy of Agricultural Sciences, the Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition,Guangdong Key Laboratory of Animal Breeding and Nutrition

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年第12期965-974,共10页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the China Agriculture Research System(No.CARS-36);the Science and Technology Program of Guangdong Province(Nos.2014A050503049 and 2013A061401020);the National Key Technology R&D Program of China(No.2012BAD39B01-5);the Science and Technology Program of Guangzhou(No.2014Y2-00121),China

摘　　要：To investigate the effect of glycitein, a synthetic soybean isoflavone（ISF）, on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF（oxidized fish oil plus ISF）. After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde（MDA）, oxidized glutathione（GSSG）, interleukin-1β（IL-1β）, tumor necrosis factor-α（TNF-α）, interleukin-2（IL-2）, nuclear factor κ B（NF-κB）, inducible nitric oxide synthase（iN OS）, NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A（sI gA） and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sI gA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa（P0.05） compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.目的:研究大豆异黄酮对氧化鱼油造成仔猪氧化应激的缓解作用,并初步探讨其作用机制。创新点:首次在仔猪肠道氧化应激模型中证明大豆异黄酮可缓解仔猪氧化应激,且此作用与提高其免疫功能相关。方法:将72头平均体重约1.8 kg的4日龄杜×(长×大)三元杂公猪随机分为3组,每组重复6次,每次重复包括4头猪:对照组(饲喂50 g/kg新鲜鱼油),处理组1(添加50 g/kg氧化鱼油)和处理组2(添加50 g/kg氧化鱼油+20 mg/kg大豆异黄酮);基础日粮根据NRC(2012)《猪的营养需要量》配制,每3小时饲喂一次,记录每天采食量;实验期21天结束后,称重,计算平均日采食量、平均日增重及料重比。用苏木精-伊红染色法观察小肠形态结构,并计算绒毛高度和隐窝深度,应用南京建成试剂盒测定肠道中抗氧化酶的活性,应用酶联免疫吸附测定(ELISA)试剂盒测定细胞炎症相关因子和细胞凋亡相关因子的表达。结论:饲喂氧化鱼油引起新生仔猪肠道产生氧化应激,引起仔猪生长性能下降、肠道发育受损、细胞凋亡增加及免疫功能失调。而添加大豆异黄酮能减缓氧化鱼油造成的肠道损伤,提高肠道粘膜完整性,提高肠道粘膜细胞抗氧化功能和免疫功能。

关 键 词：Young piglets Oxidized fish oil Growth performance Soybean isoflavone Antioxidant capacity 

分 类 号：S828.5[农业科学—畜牧学]