慢性鼻-鼻窦炎鼻息肉内镜手术前后组织中血管内皮生长因子和黏蛋白MUC5AC的表达情况  

Expression of the VEGF and mucin MUC5AC in nasal mucosa before and after chronic rhinitis-sinusitis and nasal polyps-endoscopic sinus surgery

作　　者：许航宇[1] 舒海荣[1] 宋建新[1] 

机构地区：[1]浙江省台州市中心医院耳鼻喉科,浙江台州318000

出　　处：《中国内镜杂志》2016年第12期70-74,共5页China Journal of Endoscopy

摘　　要：目的探讨慢性鼻-鼻窦炎(CRS)鼻息肉内镜手术前后鼻黏膜组织中血管内皮生长因子(VEGF)和黏蛋白MUC5AC的表达情况。方法选择2012年1月-2015年1月台州市中心医院CRS鼻息肉内镜手术患者75例作为鼻息肉组和鼻骨骨折或鼻出血患者75例作为对照组,鼻息肉患者取术前鼻息肉标本、术后6周上颌窦口黏膜标本、对照组取下鼻甲上缘黏膜标本进行HE染色观察嗜酸性粒细胞计数、免疫组化染色测定VEGF和黏蛋白MUC5AC的表达情况。结果鼻息肉术前组和鼻息肉术后组标本中嗜酸性粒细胞均高于对照组(P<0.05),鼻息肉术后组鼻黏膜嗜酸性粒细胞低于鼻息肉术前组(P<0.05)。鼻息肉术前组和鼻息肉术后组标本中VEGF和黏蛋白MUC5AC阳性表达面积百分率均高于对照组(P<0.05),鼻息肉术后组鼻黏膜VEGF和黏蛋白MUC5AC阳性表达面积百分率低于鼻息肉术前组(P<0.05)。结论嗜酸性粒细胞计数和VEGF、黏蛋白MUC5AC表达水平在CRS鼻息肉内镜术前鼻黏膜组织中较高,术后6周时明显降低,VEGF和黏蛋白MUC5AC可能参与术后鼻黏膜的修复。Objective To investigate the expression of the vascular endothelial growth factor （VEGF） and mucin MUC5AC in nasal mucosa before and after chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery.Methods 75 cases chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery patients were selected as nasal polyps group and 75 cases of nasal bone fracture or epistaxis patients as the control group from January 2012 to January 2015. Took the samples of nasal polyps before surgery and the maxillary sinus mucosa specimens after surgery six weeks of nasal polyps’ patients and on the edge of the inferior turbinate mucosa specimens of the control group to detect eosinophil count by HE staining, and detect the expression of VEGF and mucin MUC5AC by immunohistochemical staining.Results The specimens eosinophils of preoperative nasal polyp group and postoperative nasal polyp group were higher than that of control group （P〈 0.05）, the nasal eosinophils of postoperative nasal polyp group was lower than that of preoperative nasal polyp group （P〈 0.05）. The expression of specimens VEGF and mucin MUC5AC area percentages in preoperative nasal polyp group and postoperative nasal polyp group were higher than that in control group （P〈 0.05）, the expression of nasal VEGF and mucin MUC5AC area percentages in the postoperative nasal polyp group was lower than that of preoperative nasal polyp group （P〈 0.05）.Conclusion Eosinophil count and the expression levels of VEGF and mucin MUC5AC of nasal mucosa in chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery preoperative are higher, and reduce at postoperative six weeks, VEGF and mucin MUC5AC may be involved nasal repair.

关 键 词：慢性鼻-鼻窦炎 鼻内镜 鼻息肉 血管内皮生长因子 

分 类 号：R765.41[医药卫生—耳鼻咽喉科]