利用SRAP和SSR标记构建红麻遗传连锁图谱  

Constructing a Genetic Linkage Map by SRAP and SSR Marker for Kenaf( Hibiscus cannabinus L. )

作　　者：武耀龙[1] 李德芳[1] 李建军[1] 黄思齐[1] 李辉[1] 唐慧娟[1] 陈安国[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《中国麻业科学》2016年第6期249-257,267,共10页Plant Fiber Sciences in China

基　　金：基金项目 国家麻类产业技术体系(CARS-19-E07)

摘　　要：构建红麻遗传连锁图谱,为今后红麻重要农艺性状QTL定位、QTL图位克隆、优良基因筛选、分子标记辅助育种奠定良好的研究基础。对256对SRAP引物和64对棉花SSR引物进行了两次引物筛选,以泰红763×F71为作图群体亲本,150个F2代单株作为作图群体,构建红麻遗传连锁图谱。共筛选出条带清晰、多态性高的SRAP引物73对、SSR引物8对,构建的红麻遗传连锁图谱全长2155.43 c M,平均间距为16.09 c M,含有128个多态性标记,分布于18个连锁群。本研究初步证实了棉花SSR引物用于红麻是可行的,构建的红麻遗传连锁图谱密度较高,标记较为均匀,适合后续的QTL定位、QTL图位克隆等研究工作。Construction of genetic linkage map for kenaf will lay good foundation for QTL mapping, QTL map - based cloning, superior gene screening and molecular marker - assisted breeding of essential agronomic traits. 256 pairs of SRAP primers and 64 pairs of SSR primers of cotton were screened twice, 150 F2 derived from two kenaf varities, Thailand No. 1 and F71, as mapping material. 73 pairs of SRAP primers and 8 pairs of SSR primers were screened, and these primers are clearly visible and high poly- morphic. A genetic linkage map with 128 DNA loci spanning 2155.43 cM was constructed. It contains 128 polymorphic makers scattered in 18 linkage groups with average interval of genetic linkage map 16.09 cM . In this paper, it is proved that SSR primers of cotton used for kenaf are available and genetic linkage map for kenaf has relatively high density and its markers are evenly distributed, which will be suitable for QTL mapping and QTL map -based cloning and etc. in the subsequent study.

关 键 词：遗传连锁图谱 红麻 SRAP SSR 

分 类 号：S563.5[农业科学—作物学]