脐带间充质干细胞对胶质瘤细胞转移侵袭能力影响初探  

Preliminary study on impacts of UC-MSCs on metastasis and invasion of GCs

作　　者：刘高米洋[1] 朱慧[1] 何洁[1] 刘菊芬[1] 阮光萍[1] 李自安[1] 潘兴华[1] 

机构地区：[1]成都军区昆明总医院细胞生物治疗中心、干细胞与免疫细胞生物医药技术国家地方联合工程实验室、云南省细胞治疗技术转化医学重点实验室、云南省干细胞工程实验室、昆明市干细胞与再生医学研究重点实验室,昆明650032

出　　处：《西南国防医药》2016年第12期1398-1400,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家科技支撑计划项目(2014BI01B0);国家973计划项目(2012CB5181060);云南省科技计划重点项目(2013CA005)

摘　　要：目的观察人源脐带间充质干细胞(HUCMSC)对胶质瘤细胞株U87转移、侵袭能力的影响。方法建立细胞共培养体系,CCK8法、transwell法和Matrigel法分别检测HUCMSC与U87非接触共培养后,U87增殖、转移、侵袭能力变化。结果体外细胞共培养体系中,HUCMSC共培养U87组(实验组)比U87单独培养组(对照组)转移和侵袭能力都有显著提高(P<0.05),HUCMSC共培养对U87有促增殖作用。结论在体外共培养体系中,HUCMSC可能通过促进EMT转化而提高胶质瘤细胞株U87增殖、转移和侵袭潜能。因此,在HUCMSC的临床转化应用中,应该考虑其可能的促肿瘤作用。Objective To observe the impacts of human umbilical cord mesenchymal stem cells （HUC-MSCs） on the metastasis and invasion of gtioma cell （GC） line U87. Methods A cell co-culture system was established. The changes in the proliferation, metastasis and invasion of U87 after the non-contact co-culture with HUC-MSCs were detected by CCK8 method, transwell method and Matrigel method, Results The metastasis and invasion of U87 co-cultured with HUC-MSCs （in the experiment group） were much better than those of U87 separately cultured （the control group）. Conclusion In the co-culture system in vitro, HUC-MSCs may improve the proliferation, metastasis and invasion of glioma cell line U87 by promoting EMT transformation. Therefore, the potential tumor promoting effects of HUC-MSCs should be considered in the clinical transformation application.

关 键 词：脐带 间充质干细胞 胶质瘤 转移 侵袭 

分 类 号：R318.1[医药卫生—生物医学工程]