海湾扇贝维甲酸受体(RXR) cDNA克隆与表达分析  

Molecular cloning and expression of the retinoid X receptor gene in Argopecten irradians

作　　者：张瑞[1,2] 阙华勇[1] 丛日浩[1] 李莉[1] 张国范[1] ZHANG Rui QUE Hua-yong CONG Ri-hao LI Li ZHANG Guo-fan(Institute of Oceanology of the Chinese Academy of Sciences, Qingdao 266071, China University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院大学,北京100049

出　　处：《海洋科学》2016年第9期1-8,共8页Marine Sciences

基　　金：国家科技部高技术研发计划课题(863计划;2012AA10A410);贝类产业技术体系项目(CARS-48);中国科学院科技服务网络计划项目(KFJ-EW-STS-060)~~

摘　　要：维黄酸受体(RXR)是核受体超家族的重要成员,已有研究表明其在脊椎动物中广泛参与调控生长发育、新陈代谢等多种重要的生理过程。本研究采用RACE技术克隆获得海湾扇贝(Argopecten irradians)RXR(AiRXR)cDNA序列,采用荧光定量RT-PCR技术分析该基因在性腺发育不同时期的不同组织中的表达特点。结果表明,AiRXR的c DNA序列开放阅读框为1338bp,含有两个亚型:AiRXRα和AiRXRβ,两个亚型之间在T-box中相差4个氨基酸。该蛋白序列与人(Homo sapiens)和栉孔扇贝(Chlamys farreri)等的RXR相似性很高。AiRXR进化树分析显示,AiRXR与软体动物RXR聚为一支。AiRXR基因具有广泛的组织表达特点,在检测的组织中(外套膜、鳃、性腺、闭壳肌)均有表达,推测AiRXR以信号分子的形式参与多种组织细胞的生命过程;在海湾扇贝性腺发育过程中AiRXR基因在性腺发育的各时期均有表达且增殖期表达量最高,说明AiRXR基因可能参与调节性腺发育和分化的过程。The retinoid X receptor（RXR） is a member of the nuclear family of receptors. It has been proven that RXR plays a crucial role in regulating growth, development, metabolism, and other physiological process in vertebrates. Using the RACE technique, the c DNA sequence of Argopecten irradians RXR（AiRXR） was cloned. The ORF of AiRXR was 1338 bp. Two isoforms were confirmed as AiRXRα and AiRXRβ with a difference of four amino acids in the T-box. The homologous analysis of the amino acids showed that AiRXRs share a high degree of similarity with the RXRs of Homo sapiens and Chlamys farrerii. The results of q RT-PCR illustrated that the AiRXR genes are expressed in multiple tissues（mantles, gills, gonads, and adductor muscles） throughout gonadal development, indicating that AiRXRs act as one of the signal molecules involved in multiple physiological processes. The highest level of AiRXR expression was observed at the proliferation stage of gonadal development, implying that AiRXR may be involved in the regulation of gonadal development and differentiation.

关 键 词：海湾扇贝(Argopecten irradians) RXR基因 组织表达 性腺发育 

分 类 号：Q786[生物学—分子生物学]