角类肥蛛体内共生菌多样性检测  

The diversity of endosymbionts in Larinioides cornuta

作　　者：苏其琛 刘劲涛[1] 彭宇[1] 云月利[1] SU Qi-Chen LIU Jin-Tao PENG Yu YUN Yue-Li(Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Centre for Behavioral Ecology and Evolution, College of Life Sciences, Hubei University, Wuhan 430062, P. R. Chin)

机构地区：[1]生物资源绿色转化湖北省协同创新中心、行为生态与进化中心、生命科学学院、湖北大学,武汉430062

出　　处：《蛛形学报》2016年第2期111-114,共4页Acta Arachnologica Sinica

基　　金：国家自然科学基金（31401982、31672317）,湖北省对外科技合作项目（2016AHB003）资助项目.

摘　　要：本研究以角类肥蛛（Larinioides cornuta）为实验材料，采用DNA提取试剂盒提取角类肥蛛的基因组DNA，把10头角类肥蛛的基因组DNA按照等摩尔量混合，以混合DNA为模版，用细菌16SrDNA的1对通用引物27F和1487R进行PCR检测并进行序列测定。检测结果：角类肥蛛只感染了Spiraplasma1个属的内共生菌，50个克隆测序结果共获得11个不同的Spiraplasma序列（KX559370．KX559380）。所获得的序列与NCBI核苷酸库的比对结果显示，角类肥蛛体内检测到的Spiraplasma与鞘翅目的1种象鼻虫（ Curculio elephas ）和瓢甲科Anisosticta属昆虫体内检测到的Spiraplasma相似度较高（98％-99％）；所获序列与NCBI核糖体RNA数据库的比对结果显示，角类肥蛛体内的Spiraplasma共生菌与Spiroplasmaixodetis相似度最高（98％-99％）。In this study, L. cornuta was used for the extraction of genome DNA. 10 individual DNA of L. cornuta were blended according to the same DNA molar quality. A target fragment was amplified with the template of blended DNA, and the primer was 27F and 1487R of 16S r DNA. The results showed that only Spiroplasma was detected in L. cornuta, and 11 sequences（KX559370-KX559380） of Spiroplasma were obtained. The result of blast with NCBI nucleotide collection showed the Spiroplasma from L. cornuta was similar to the Spiroplasma from Curculio elephas and the insects of genus Anisosticta （98%-99%）. The result of blast with NCBI 16S ribosomal RNA of GenBank showed Spiroplasma obtained in this study was similar to Spiroplasma ixodetis（97%-98%）.

关 键 词：角类肥蛛 内共生菌 多样性 

分 类 号：Q959.226[生物学—动物学]