梭梭和柽柳土壤微生物多样性初步分析  

Phylogenetic Diversity Preliminary Analysis of Haloxylon ammnodendron and Tamarix ramosissima Soil Bacteria

作　　者：杨瑞红[1,2,3] 赵成义[2] 王新军[3] 马亚丽[1] YANG Ruihong＇＇ ZHAO Chengyi. WANG Xinjun MA Yali(Xinjiang Education Institute, Urumqi 840043, China Xingjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China Xijiang Agriculture University, Urumqi 830052, China)

机构地区：[1]新疆教育学院,乌鲁木齐830043 [2]中国科学院新疆生态与地理研究所,乌鲁木齐830011 [3]新疆农业大学,乌鲁木齐830052

出　　处：《土壤》2016年第6期1120-1130,共11页Soils

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB429905);国家自然科学基金项目(41301205;2014211B014)资助

摘　　要：直接从沙漠土壤中提取混合微生物DNA，利用Illuminamiseq测序平台，对16SrDNA进行测序和分析。结果表明：①荒漠植被土壤微生物数量很少，生物活性极弱，DNA提取难度大；②沙漠植被土壤细菌多样性丰富，所有测得细菌分属到23个门和316个属，其中还存在一定数量的微生物新种，部分测得代表新属和种的序列提交GenBank，获得序列号（KT984242～KT984249）；③不同沙漠土壤样品微生物群落有相似性，但也有较明显的差异，其差异与土壤理化因子等因素有关：如土壤含水量与酸杆菌和变形菌的分布相关；相对于空地，梭梭和柽柳群落土壤独有的迷踪菌门（Elusimicrobia）与固氮菌密切相关；梭梭的土壤环境pH明显高于柽柳土壤环境这决定了它们不同的优势种；土壤微生物生物量碳的大小不能反映微生物量的种类多少，但可以反映微生物数量的多少。Microbiological mixed DNA （genomic DNA） was directly extracted from soil in the Gurbantunggut desert. Using Illuminamiseq sequencing platform, diversity of community structure was analyzed from the bacterial 16S rDNA. The research showed that： 1） Because of the low quantity of microbe in desert vegetation soil and the low activity, there were many difficulties in the DNA extraction; 2） In total, measured sequences belonged to 23 phyla and 316 genera, among them, some sequences were uncultured groups that had an uncertain affiliation, which had been submitted to the GenBank PEMBL PDDBJ databases under accession numbers KT984242-KT984249.3）There were some discrepancies between groups. The difference was affected by factors such as soil physical and chemical factors. The water content influenced the amount of Proteobacteria and Acidobacteria. The unique Elusimicrobia was the soil of plant Haloxylon ammnodendron and Tamarix ramosissima, which was closely related to the nitrogen fixing bacteria. The soil microbial biomass carbon could reflect the quantity of microorganism but not the types of microorganism.

关 键 词：梭梭 柽柳 DNA提取 土壤微生物多样性 系统发育分析 

分 类 号：Q938[生物学—微生物学]