电针心经腧穴对急性心肌缺血大鼠自主神经活动的影响  

Effect of Electroacupuncture Stimulation of Heart Meridian on Autonomic Nervous Activities in Acute Myocardial Ischemia Rats

作　　者：崔帅[1] 许静[1] 王洁[1] 吴生兵[1] 周逸平[1] 周美启[1] CUI Shuai XU Jing WANG Jie WU Sheng-bing ZHOU Yi-ping ZHOU Mei-qi(InstituteofAcu-moxibustion and Meridian, Anhui University of Traditional Chinese Medicine, Hefei 230012, China)

机构地区：[1]安徽中医药大学针灸经络研究所,合肥230012

出　　处：《针刺研究》2016年第6期515-520,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81273858);安徽高校科研创新平台团队建设项目(No.2015TD 033);安徽省学术和技术带头人及后备人选科研活动经费资助项目(No.20145D070);安徽高校省级自然科学研究项目(No.KJ 2016A857);安徽省自然科学基金项目(No.1408085QH 164)

摘　　要：目的:观察电针心经"神门-通里"经脉段对急性心肌缺血大鼠交感神经和迷走神经放电的影响,探讨两者在电针心经改善急性心肌缺血中的协同调节作用。方法:SD大鼠随机分为伪手术组、模型组和电针心经组,每组10只。冠状动脉左前降支结扎法复制大鼠急性心肌缺血模型。电针心经组给予电针刺激大鼠心经"神门-通里"经脉段,刺激20min。用两根铂金丝电极分别同步引导左侧交感神经和迷走神经,同时记录模型复制前5min,结扎后即刻,电针后1、3、5、15 min的心电图、交感神经和迷走神经放电频率。结果:急性心肌缺血模型复制后,与伪手术组比较,模型组大鼠各时相心率、心电图J点电压、交感神经放电频率显著升高(P<0.01),迷走神经放电频率显著降低(P<0.01);与模型组比较,电针心经组大鼠各时相心率、心电图J点电压、交感神经放电频率均显著降低(P<0.01),迷走神经放电频率显著升高(P<0.01)。结论:电针心经可通过同时抑制交感神经放电和促进迷走神经放电,发挥改善急性心肌缺血的协同调节作用。Objective To observe the effect of electroacupuncture（EA）stimulation of the Heart Meridian on electrical activities of neck sympathetic and vagal nerves in acute myocardial ischemia（AMI）rats.Methods Thirty SD rats were randomly divided into 3groups,sham operation（sham）,model and EA（n=10rats in each group）.The left anterior descending branch（LADB）of the coronary artery was occluded to make an acute myocardial ischemia（AMI）model.For rats of the sham group,a surgical suture was simply threaded beneath the LADB without ligation.EA stimulation（2Hz,1mA）was applied to bilateral＂Shenmen＂（HT 7）,＂Tongli＂（HT 5）and the middle-point between HT 7and HT 5for 20 min.The electrical activities of the left cervical sympathetic and vagal nerves were recorded using a bipolar platinum wire electrodes and a BIOPAC MPl 50 system,and the electrocardiogram（ECG）of the standard limb lead II was recorded simultaneously.The AMI severity was evaluated by using J-spot height of ECG.Results After AMI,the heart rate（HR）,J-spot of ECG,and the discharge frequency of the cervical sympathetic nerve were significantly increased in the model group compared with the sham group（P〈0.01）,while the discharge frequency of vagal nerve was obviously decreased（P〈0.01）.Following EA stimulation,the increased levels of HR,J-spot height and discharge frequency of sympathetic nerve,and the decreased discharge frequency of vagus were significantly suppressed at time-points of 1,3,5and 15 min after EA（P〈0.01）.In addition,the ratio of discharge frequencies of vagal/sympathetic nerves was notably down-regulated after AMI（P〈0.01）.After EA stimulation,the ratios of vagal/sympathetic discharge at the time-points of 1,3,5and 15 min following EA were significantly increased（P〈0.01）.Conclusion EA stimulation of the Heart Meridian may improve AMI by inhibiting sympathetic discharges and elevating vagal discharges in AMI rats,i.e.,coordinating activities of the autonomic nerve systems.

关 键 词：急性心肌缺血 电针 心经 交感神经 迷走神经 

分 类 号：R245.97[医药卫生—针灸推拿学]