针刺对卒中后抑郁大鼠脑组织神经递质及5-羟色胺转运体、5-羟色胺1A受体、去甲肾上腺素α2受体的影响  

Effect of Manual Acupuncture Intervention on Levels of 5-HTT,5-HT_(1A)R,NEα_2R of Brain Tissues in Rats with Post-stroke Depression

作　　者：肖伟[1] 章显宝[1] 王震[1] 汪燕[2] 郭晓利[1] 何玲[2] 梁发俊[1] 胡徽星[1] XIAO Wei ZHANG Xian-bao WANG Zhen WANG Yan GUO Xiao-li HE Ling LIANG Fa-jun HU Hui- xing(The Second Affiliated Hospital, Anhui University of Chinese Medicine, Hef ei 230061 , China Anhui Medical University, Hefei 230038)

机构地区：[1]安徽中医药大学第二附属医院,合肥230061 [2]安徽中医药大学,合肥230038

出　　处：《针刺研究》2016年第6期528-534,共7页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81273857)

摘　　要：目的:观察解郁安神针法对卒中后抑郁(PSD)大鼠脑组织5-羟色胺(5-HT)转运体(5-HTT)、5-HT 1A受体(5-HT_(1A)R)、去甲肾上腺素α2受体(NEα_2R)蛋白的影响。方法:将SD大鼠随机分为正常组、模型组、药物组、针刺组,每组各10只。采用大脑中动脉闭塞和慢性不可预见性温和性应激法复合制备卒中后抑郁模型。药物组予以氟西汀(2mg/kg)灌胃,针刺组选取"百会""风府"及双侧"神门""太冲"穴针刺20min,每日1次,7d为1个疗程,共3个疗程,疗程之间休息1d。观察大鼠行为学并采用高效液相色谱法检测大脑皮质5-HT、去甲肾上腺素(NE)、乙酰胆碱(Ach)、γ-氨基丁酸(γ-GABA)、谷氨酸(Glu)变化,采用免疫印迹法检测海马、中缝核、蓝斑核5-HTT、5-HT_(1A)R、NEα_2R蛋白变化。结果:与正常组大鼠比较,PSD模型大鼠大脑皮质5-HT、NE及海马、中缝核、蓝斑核中5-HTT蛋白表达降低,大脑皮质Ach、γ-GABA、Glu及海马、中缝核、蓝斑核中NEα_2R、5-HT_(1A)R蛋白表达升高(P<0.01);与模型组大鼠比较,针刺组脑皮质5-HT、NE、5-HTT蛋白表达升高(P<0.01),Ach、γ-GABA、Glu、NEα_2R、5-HT_(1A)R蛋白表达降低(P<0.01,P<0.05)。结论:解郁安神针法能明显改善PSD大鼠行为学变化,其机制可能是上调大脑皮质5-HT、NE,下调Ach、γ-GABA、Glu表达,促进海马、中缝核、蓝斑核5-HTT敏感性,抑制NEα_2R、5-HT_(1A)R表达。Objective To observe the effect of manual acupuncture intervention on serotonin transporter（5-HTT）,5-HT1Areceptor（5-HT1AR）,norepinephrine（NE）alpha receptor 2（NEα2R）protein of brain tissues in rats with post-stroke depression（PSD）.Methods Forty SD rats were randomly divided into normal group,model group,medication group and acupuncture group,with 10 rats in each.The PSD model was established by the combination of middle cerebral artery occlusion and chronic non-predictable mild stress.Rats of the medication group were treated by gavage of fluoxetine（2 mg/kg,once daily for 21days）,and those of the acupuncture group treated by manual acupuncture stimulation of＂Baihui＂（GV 20）,＂Fengfu＂（GV 16）,bilateral＂Shenmen＂（HT 7）and＂Taichong＂（LR 3）for 20 min,once a day for 21 days,with one day＇s interval between each every7 days.The animals＇ neurological deficit was detected by Zea Longa＇s scaling,and the depression status assessed by sugar solution consumption,and the locomotor activity（crossing and rearing times in 5min）detected by open-field tests.The contents of 5-HT,NE,acetylcholine（ACh）,gamma aminobutyric acid（GABA）,and glutamate（Glu）of the cerebral cortex were detected using high performance liquid chromatography（HPLC）and expression levels of 5-HTT,5-HT1 AR,NEα2R proteins of the hippocampus,raphe nuclei and locus coeruleus（LC）tissues detected using western blotting（EB）,respectively.Results After modeling,the neurological deficit score was significantly increased,while the sugar solution consumption and the locomotor activities（crossing and rearing times in 5min）were notably decreased（P〈0.05,P〈0.001）.Findings of HPLC and WB indicated that after modeling,the cerebrocortical 5-HT and NE contents and 5-HTT protein expression levels in the hippocampus,raphe nucleus and LC were obviously decreased and the cortical ACh,GABA and Glu contents as well as the expression of 5-HT1 AR and NEα2R proteins in the hippocampus,raphe nucleu

关 键 词：卒中后抑郁 针刺干预 5-羟色胺转运体 5-羟色胺1A受体 去甲肾上腺素α2受体 大脑皮质 海马 中缝核 蓝斑核 

分 类 号：R245.311[医药卫生—针灸推拿学]