恶性淋巴瘤诊断中荧光原位杂交分析基因状态的研究  

Genetic status analysis of 87 cases in malignant lymphoma diagnosis fluorescence in situ hybridization

作　　者：沈小英[1] 亓岽东[2] 武鑫瑞[1] 屈悦 张立英[4] 张玉萍[5] 许春伟[6] 吴继华[1] Shen Xiaoying Qi Dongdong Wu Xinrui Qu Yue Zhang Liying Zhang Yuping Xu Chunwei Wu Jihua(Department of Pathology, 306 Hospital of PLA , Beijing 100101 , China Department of On- cology , Zhongshan Hospital, Dalian University, Dalian 116001 ,China Beijing Xinji Yongkang Gene Sci- ence and Technology Co. , Ltd. , Beijing 100085, China Department of Pathology, The Military General Hospital of Beijing PLA, Beijing 100700 ,China Department of Pathology, The People＇s Hospital of Wei- fang, Wei fang , Shandong 261041 ,China Department of Pathology, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071 , China.)

机构地区：[1]解放军306医院病理科,北京100101 [2]大连大学附属中山医院肿瘤科,辽宁大连116001 [3]北京新基永康基因科技有限公司,北京100085 [4]北京军区总医院病理科,北京100700 [5]山东省潍坊市人民医院病理科,山东潍坊261041 [6]军事医学科学院附属医院病理科,北京100071

出　　处：《贵州医药》2016年第11期1126-1128,F0003,共4页Guizhou Medical Journal

摘　　要：目的探讨恶性淋巴瘤中基因重排的特点。方法回顾性分析87例恶性淋巴瘤组织样本,利用荧光原位杂交技术,分别检测弥漫大B细胞淋巴瘤、滤泡性淋巴瘤、黏膜相关淋巴组织淋巴瘤、套细胞淋巴瘤、Burkitt淋巴瘤和间变性大细胞淋巴瘤中BCL-6、BCL-2、MALT1、CCND1、C-MYC和ALK基因重排。结果弥漫大B细胞性淋巴瘤中BCL-6基因重排的阳性率为29.03%(9/31),滤泡性淋巴瘤中BCL-2基因重排的阳性率为61.11%(11/18),黏膜相关淋巴组织淋巴瘤中MALT1基因重排的阳性率为47.37%(9/19),套细胞淋巴瘤中CCND1基因重排的阳性率为88.88%(8/9),Burkitt淋巴瘤中C-MYC基因重排的阳性率为100%(2/2)和间变性大细胞淋巴瘤中ALK基因重排的阳性率为75.00%(6/8)。结论基因重排对恶性淋巴瘤的诊断具有重要的实际价值。Objective To analyze the characteristics of gene rearrangement in malignant lymphoma.Methods A retrospective analysis of 87 cases of samples with malignant lymphoma,which were used to detect gene rearrangement by fluorescence in situ hybridization,and Subtypes of diffuse large B cell lymphoma with the BCL-6gene rearrangement,follicular lymphoma with the BCL-2gene rearrangement,the mucosa associated lymphoid tissue lymphoma with MALT1 gene rearrangement,mantle cell lymphoma with CCND1 gene rearrangement,Burkitt lymphoma with C-MYC gene rearrangement and anaplastic large cell lymphoma with ALK gene rearrangement,respectively.Results The positive rate of BCL-6gene rearrangement was 29.03%（9/31）in diffuse large B cell lymphoma,the positive rate of BCL-2gene rearrangement was 61.11%（11/18）in follicular lymphoma,the positive rate of MALT1 gene rearrangement was 47.37%（9/19）in mucosa associated lymphoid tissue lymphoma,the positive rate of CCND1 gene rearrangement was 88.88%（8/9）in mantle cell lymphoma,the positive rate of C-MYC gene rearrangement was 100%（2/2）in Burkitt lymphoma and the positive rate of ALK gene rearrangement was 75.00%（6/8）in anaplastic large cell lymphoma.Conclusion Gene rearrangement has an important practical value to the diagnosis of malignant lymphoma.

关 键 词：淋巴瘤 BCL-6基因 BCL-2基因 MALT1基因 CCND1基因 C-MYC基因 ALK基因 

分 类 号：R714.55[医药卫生—妇产科学]