镉对果蝇金属硫蛋白表达的影响  

Effect of cadmium on the expression of metallothionein in Drosophila melanogaster

作　　者：杜移珍 梁露[1] 付伟利[1] 叶海燕[1] 张敏[1] DU Yi-zhen LIANG Lu FU Wei-li YE Hai-yan ZHANG Min(College of Life Sciences, Shaanxi Normal University, Xi＇an 710062, China)

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《安全与环境学报》2016年第6期383-387,共5页Journal of Safety and Environment

基　　金：国家自然科学基金项目(31201762);中央高校基本科研业务费专项(GK201402026)

摘　　要：以黑腹果蝇Canton-S品系为试验材料,采用镉饱和法测定重金属镉胁迫后果蝇金属硫蛋白质量比的变化,并应用实时荧光定量PCR(Real-Time PCR)方法检测了不同质量浓度镉处理后果蝇金属硫蛋白相关基因MtnB、MtnC、MtnD、MtnE及金属耐受因子Hmt-1基因mRNA水平的表达差异。结果表明,1)不同质量浓度镉处理对雌、雄果蝇均产生明显的生理影响,导致果蝇寿命缩短。镉胁迫果蝇10 d后,其半数致死质量浓度为52.0 mg/L,为后期果蝇对镉胁迫产生的解毒机制研究提供浓度设定依据。2)镉胁迫后雌、雄果蝇体内金属硫蛋白质量比显著升高,与对照组相比差异显著,并存在一定的剂量依赖性;3)镉胁迫导致黑腹果蝇金属硫蛋白相关基因mRNA水平表达上调,并具明显的剂量依赖性,同时在一定范围内Hmt-1基因的表达随镉质量浓度升高而上调,具有浓度依赖效应。The aim of this paper is to present the study results on the responsive reactions and the detoxification mechanisms of cadmium （ one of the major heavy metal contaminants in the environment） in Drosophila melanogaster （Canton- S）, one of the essential model organisms in biological research area. For the study purpose, we have investigated and tested the mortality of Drosophila melanogaster after being fed with Cd （1. 875 mg/L, 3.75 mg/L, 7.5 rag/L, 15.0 mg/L, 30. 0 mg/L, 60. 0 mg/L, 90. 0 rag/L） for 10 d. The results of our testing have shown that cadmium can reduce the lifespan of Drosophila melanogaster both for males and females, with the median lethal mass concentration of Drosophila inelanogaster being 52. 0 mg/L, which has resulted in a significant reference for further researches. And, then, we have made an evaluation of the metallothionein contents and the expression of different genes, namely MtnB, MtnC, MtnD, MtnE and Hint - 1 （ heavy metal tolerance factor 1 ） in case when Drosophila melanogaster was exposed to cadmium with different mass concentrations （6.5 mg/L, 13.0 mg/L, 26.0 mg/L, 52.0mg/L）, with the saturation method and Real-Time PCR. The results we have gained through the tests indicate that, as compared with the control group, the metallothionein contents have shown a significant increase in the cadmium treatment groups with a dose-dependent responsive response （p 〈 0. 05）. Besides, the expression of the metallothionein genes （MtnB, MtnC, MtnD, MtnE） tends to be significantly up-regulated, with a significant difference as compared with the control group （p 〈0. 05 ）. What is the most important for our research is that, with the increase of cadmium concentration, the expression of Hint - 1 gene gets gradualist up-regulated （p 〈 0. 05 ） , which is supposed to prove that Drosophila melanogaster have gained somewhat resistant power against the heavy metal toxicity by their own responsive physiological mecha- nisms, such as regulating the expression of meta

关 键 词：环境科学技术基础学科 镉 黑腹果蝇 金属硫蛋白 金属耐受因子 

分 类 号：X503.223[环境科学与工程—环境工程]