白藜芦醇对哮喘小鼠磷脂酶A_2活性和气道炎症的影响  

Effect of resveratrol on the PLA_2 activity and airway inflammation in asthmatic mice

作　　者：王经韵[1] 邱心敏 罗夏炯 陈小夏[2] 何冰[2] WANG Jingyun QIU Xinming LUO Xiajiong CHEN Xiaoxia HE Bing(Guangdong General Hospital, Guangzhou 510080, China College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China)

机构地区：[1]广东省人民医院国家临床药物实验机构办公室,广东广州510080 [2]广东药科大学药学院,广东广州510006

出　　处：《广东药学院学报》2016年第5期606-608,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科学技术厅省中医药科学院联合科研专项项目(粤科社字[2013]102号)

摘　　要：目的探讨白藜芦醇对哮喘小鼠磷脂酶A_2活性和气道炎症的影响。方法采用卵清蛋白(OVA)刺激,建立小鼠哮喘模型,测定哮喘小鼠支气管肺泡灌洗液(BALF)中白细胞总数及嗜酸粒细胞(EOS)计数;紫外分光光度法测定肺组织中前列腺素E2(PGE2)的含量;盐酸滴定法测定肺组织和血清中磷脂酶A_2活性。结果白藜芦醇能显著减少哮喘模型小鼠BALF中白细胞总数及EOS计数,减少肺组织中PGE2含量,降低磷脂酶A_2活性。结论白藜芦醇对哮喘小鼠有保护作用,可能与其降低磷脂酶A_2活性、减轻炎性细胞浸润、减少炎症介质PGE2的产生有关。Objective To investigate the effect of resveratrol on the PLA2 activity and airway inflammation in asthmatic mice. Methods The asthmatic model was induced by ovalbumin provocation in mice. The number of total leukocytes and eosinophils（ EOS） in bronchoalveolar lavage fluid（ BALF） was counted. The PGE2 content in lung tissue was measured by ultraviolet spectrophotometry. The PLA2 activities of lung tissue and serum were assayed by the hydrochloric acid titration method. Results The inflammatory cells in BALF,the PGE2 content and the PLA2 activity in resveratrol-treated group were significantly lower than that of the model group. Conclusion Resveratrol displays a protective effect in asthmatic mice,which may be related to reduction of the inflammatory cell infiltration,the PGE2 content and the PLA2 activity.

关 键 词：白藜芦醇 支气管哮喘 炎症细胞 前列腺素E2 磷脂酶A2 

分 类 号：R562.25[医药卫生—呼吸系统]