3起GⅡ.17型诺如病毒突发疫情的分子流行病学分析  

Molecular epidemiological analysis on 3 outbreaks of GⅡ. 17 norovirus

作　　者：许金凤[1,2] 徐虹[2] 薛渊[2] 周印[2] 邵世和[1] 申红星[1] XU Jin- feng XU Hong XUE Yuan ZHOU Yin SHAO Shi -he SHEN Hong- xing(Jiangsu University, Zhenjiang, Jiangsu 212013, China)

机构地区：[1]江苏大学,江苏镇江212013 [2]镇江市疾病预防控制中心,江苏镇江212000

出　　处：《中国卫生检验杂志》2016年第23期3423-3425,3428,共4页Chinese Journal of Health Laboratory Technology

基　　金：镇江市卫生科技重点专项项目(SHW2015015)

摘　　要：目的了解3起学校诺如病毒突发疫情的分子流行病学特征,分析诺如病毒疫情的基因型。方法采用实时荧光定量反转录PCR检测标本诺如病毒RNA,阳性标本进行逆转录PCR扩增、电泳、测序和进化树分析。结果 3起学校疫情的17份肛拭子样本中,诺如病毒GⅡ型阳性12份。10株疫情病毒经序列测定和比对,均为GⅡ.17亚型;与来自Gen Bank数据库的GⅡ.17亚型NV参考株相似性均在98%以上;系统发生树上与GⅡ.17基因亚型在一个进化分支上,属于GⅡ.17基因亚型。结论 GⅡ.17型诺如病毒将是今后疾病预防控制机构进行长效监测和防控的重点。Objective To understand the molecular epidemiological characteristics of three cases of norovirus outbreaks in school and analyze the genotypes of norovirus. Methods Real-time fluorescent RT-PCR was used to detect RNA of the samples,and the positive samples were conducted for RT-PCR amplification,electrophoresis,sequencing and phylogenetic tree analysis. Results In 17 anal swab samples from 3 school outbreaks,12 samples were norovirus GⅡ type. 10 strains of virus,by sequencing and comparison,were GⅡ. 17 subtype; similarity with GⅡ. 17 reference strains from Gen Bank database were more than 98%; basing on GⅡ. 17 subtypes evolutionary branch in phylogenetic tree,belonging to GⅡ. 17 subtype. Conclusion Suggesting that GⅡ. 17 will be the focus of long-term monitoring and prevention for disease control and prevention institutions in the future.

关 键 词：诺如病毒 GⅡ.17型 进化树分析 

分 类 号：Q939.4[生物学—微生物学]