白芍总苷脂质体诱导胃癌BGC-823细胞凋亡的实验研究  

Apoptosis of Human Gastric Cancer Cell Line BGC-823 Induced by Liposome of Total Glucosides from Paeonia

作　　者：鲍舒洁 张丹 张红 孙玉红 刘明华 乔西 BAO Shu-jie ZHANG Dan ZHANG Hong SUN Yu-hong LIU Ming-hua QIAO Xi(School ofPhamacology, South- west Medical University, Luzhou 646000, Chin)

机构地区：[1]西南医科大学药学院,四川泸州646000

出　　处：《中国药学杂志》2016年第24期2109-2113,共5页Chinese Pharmaceutical Journal

基　　金：四川省科技厅资助项目(2015JY0098);西南医科大学大学生创新创业训练计划(201388)

摘　　要：目的探讨白芍总苷脂质体对人胃癌BGC-823细胞凋亡的诱导作用。方法 CCK-8试剂盒检测白芍总苷脂质体对BGC-823细胞增殖的影响,HE染色、Hochest 33342/PI双染和透射电子显微镜观察BGC-823细胞凋亡的形态学变化,Western blotting检测细胞凋亡相关因子Bcl-2和Bax蛋白的表达情况。结果白芍总苷脂质体能明显抑制BGC-823细胞的增殖,呈现一定的剂量依赖关系(P<0.05)。白芍总苷脂质体干预BGC-823细胞48 h后,凋亡形态学观察均可见细胞核固缩、碎裂和凋亡小体等凋亡特征改变,Western blotting检测Bcl-2蛋白表达减少,Bax表达增多,Bcl-2/Bax比值降低。结论白芍总苷脂质体明显诱导胃癌BGC-823细胞凋亡,抑制其增殖,其机制可能与下调Bcl-2蛋白、上调Bax蛋白及降低Bcl-2/Bax比值有关。OBJECTIVE To study the apoptosis of human gastric cancer cell line BGC-823 induced by liposome of total gluco- sides from paeonia （TGP liposome ）. METHODS The inhibition of cell proliferation was determined by Cell Counting Kit-8 as- say. The morphological changes of the apoptosis cells were observed by HE staining, Hoechst 33258 fluorescent staining, transmission e- lectron microscope. Changes of apoptosis related factor Bcl-2 and Bax protein expression were detected by Western blot. RESULTS TGP liposome significantly inhibited proliferation of BGC-823 cells in a dose-dependent manner（ P 〈 0. 05 ）. GC-823 cells induced by TGP liposome for 48 h was observed on morphological changes such as karyopyknosis and karyorrhexis and apoptotic bodies. Bel-2 ex- pression was reduced, but Bax expression was increased, so the ratio of Bcl-2 /Bax decreased. CONCLUSION TGP liposome can induce the apoptosis of BGC-823 cells and inhibit its proliferation, its mechanism may be related to down-regulating protein expression of Bel-2, up-regulating protein expression of Bax, reducing Bcl-2/Bax value.

关 键 词：白芍总苷脂质体 BGC-823 凋亡 BCL-2/BAX 

分 类 号：R285.5[医药卫生—中药学]