白术醇提物的抗炎镇痛活性研究  

Study on Anti-inflammatory and Analgesic Effect of Ethanol Extract of Atractylodes Macrocephala Koidz.

作　　者：赵桂芝[1,2] 浦锦宝[1,2] 周洁[1,2] 梁卫青[1,2] 胡轶娟[1,2] 张宏建[1,2] 徐攀[1,2] ZHAO Guizhi PU Jinbao ZHOU Jie LIANG Weiqing HU Yijuan ZHANG Hongjian XU Pan(Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China)

机构地区：[1]浙江省中医药研究院,杭州310007 [2]浙江省中药新药研发重点实验室,杭州310007

出　　处：《中国现代应用药学》2016年第12期1507-1512,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技计划项目(2008F3036);浙江省中医药科技计划项目(2013ZA006)

摘　　要：目的探讨白术醇提物的抗炎镇痛活性。方法采用热板法测定白术不同剂量组小鼠的痛阈值,腹腔注射0.6%醋酸刺激致痛模型（扭体法）观察白术3个不同剂量的镇痛作用;采用二甲苯致小鼠耳廓肿胀实验和角叉菜胶致大鼠足肿胀实验观察3个不同剂量白术的抗炎作用。结果高、中剂量的白术醇提物可显著增加小鼠的热板痛阈值（P〈0.01）,减少腹腔注射醋酸引起的小鼠扭体反应次数（P〈0.01）,而白术低剂量组不能有效的提高小鼠的痛阈值和减少扭体反应次数。在抗炎试验中,高、中剂量的白术醇提物可显著抑制小鼠耳廓肿胀度（P〈0.01）,而低剂量组对小鼠耳廓肿胀抑制效果不明显;高剂量组在2 h后能显著抑制大鼠足跖肿胀,中剂量组（除6 h时间点）与白术醇提物低剂量组（除3 h时间点）在药后0.5-6 h之间与模型组比较均无显著性差异。结论白术醇提物具有较好的抗炎、镇痛作用,并呈现一定的剂量依赖性。OBJECTIVE To investigate the analgesic and anti-inflammatory activities of the ethanol extract of Atractylodes macrocephala Koidz.（EAM）. METHODS Analgesic activity of EAM was evaluated by hot-plate test and acetic acid-induced writhing test on ICR mice, while anti-inflammatory activity of EAM was assessed by xylene-induced ear edema on ICR mice and carrageenan-induced rat paw edema on SD rats. RESULTS High and medium doses of the EAM could prolong the threshold time of pain（P〈0.01）, reduce the writhing times induced by acetic acid（P〈0.01）, and inhibit the edema induced by xylene and canageenan（P〈0.01）, while low dose of the EAM showed no siginificance differences compared to the control group in all experiments. In carrageenan-induced paw edema model, high dose of EAM could inhibit the paw swelling significantly since 2 h of administration, and in the period of 0.5？6 h after drug administration, medium dose of EAM（with the exception of 6 h） and low dose of EAM（with the exception of 3 h） had no significant differences compared with model group. CONCLUSION The ethanol extract of EAM has analgesic and anti-inflammatory effects, and present the dose-response relationship.

关 键 词：白术 醇提物 抗炎 镇痛 

分 类 号：R285.5[医药卫生—中药学]