IL-6、IL-17、IL-18及MCP-1联合检测B型柯萨奇病  

Diagnostic value of combination detection of IL-6, IL-17, IL-18 and MCP-1 on CoxB-VMC

作　　者：黄凯达[1] 王仕海[2] 朱庆华[1] 毛红丽[1] 

机构地区：[1]郑州大学第一附属医院检验科,450052 [2]郑州市第一人民医院检验科

出　　处：《中国实用医刊》2016年第23期1-3,共3页Chinese Journal of Practical Medicine

摘　　要：目的探讨B型柯萨奇病毒性心肌炎（CoxB-VMC）患者血清白细胞介素-6（IL-6）、白细胞介素-17（IL-17）、白细胞介素-18（IL-18）及单核细胞趋化蛋白-1（MCP-1）水平的变化及联合检测对CoxB-VMC临床诊断的价值。方法选取VMC患者158例为实验组，健康体检者94例为对照组。采用酶联免疫吸附法（ELISA）测定两组患者血清IL-6、IL-17、IL-18、MCP-1的含量及B型柯萨奇病毒（CoxB）IgM抗体，电化学发光法检测两组血清中肌酸激酶同工酶（CK-MB）及心肌肌钙蛋白T（cTnT）的水平，分析各指标的水平与CoxB-VMC的关系，评价IL-6、IL-17、IL-18及MCP-1联合检测对CoxB-VMC诊断的价值。结果AVMC急性期血清CK-MB、cTnT、IL-6、IL-17、IL-18及MCP-1水平分别为（27.90±4.72）、（0.37±0.02）、（361.60±45.39）、（445.70±67.97）、（168.60±35.87）、（376.20±49.50）ng/L，显著高于对照组（P〈0.01）；VMC患者CoxB病毒感染与心脏功能分级、IL-6、IL-17、IL-18及MCP-1水平呈正相关（P〈0.05）。IL-6、IL-17、IL-18及MCP-1联合检测可显著提高CoxB-VMC检测的敏感性（97.30%）、特异性（94.60%）及准确率（96.70%）。结论VMC急性期IL-6、IL-17、IL-18及MCP-1水平变化在一定程度上可反映CoxB-VMC的心脏功能状态，该4种指标的联合检测可增强CoxB-VMC的临床诊断和病情评估。Objective To determine the levels of serum IL-6, IL-17, IL-18 and MCP-1 and the diagnostic value of the combination detection of IL-6, IL-17, IL-18 and MCP-1 on CoxB-VMC. Methods The 158 patients with VMC were selected as the experiment group and 94 healthy persons were selected as the control group. The serum CK-MB and cTnT were tested by electrochemolumines- cence assay, while the levels of IL-6, IL-17, IL-1, MCP-1 and CoxB IgM antibody were detected by ELISA assay. Results The serum levels of CK-MB, cTnT, IL-6, IL-17, IL-18 and MCP-1 in patients with VMC were （27. 90±4. 72）, （0. 37±0. 02）, （361.60±45.39）, （445.70±67.97）, （ 168.60 ±35.87 ） and （376.20 ~ 49.50） ng/L respectively, which were significantly higher than those of healthy persons （P 〈0.01 ）. The CoxB virus infection in VMC patients was positively correlated with tlae heart function classification, IL-17, IL-18, IL-6 and MCP-1 levels （P 〈 0. 05 ）. The combined detection of IL-6, IL-17, IL-18 and MCP-1 can significantly improve the sensitivity （97.30%）, specificity （94.60%） and accuracy （96.70%）of CoxB-VMC detection. Conclusions The serum levels of IL-6, IL-17, IL-18 and MCP-1 in acute phase can reflect the cardiac function of patients with CoxB-VMC in certain extent, and the combined detection of these four indicators can effectively enhance the clinical di- agnosis and assessment of CoxB-VMC.

关 键 词：B型柯萨奇病毒性心肌炎 白细胞介素-6 白细胞介素-17 白细胞介素-18 单核细胞趋化蛋白-1 联合检测 

分 类 号：R542.21[医药卫生—心血管疾病]