粘蛋白在胆固醇结石和胆囊息肉胆囊组织中的表达和分泌差异  

Expression and Secretion of Mucin in Cholesterol Gallstone Disease versus Gallbladder Polyps

作　　者：崔小红 CUI Xiaohong(Department of General Surgery, Shanghai Dianli Hospital, Shanghai, 200050, Chin)

机构地区：[1]上海电力医院普外科,上海市200050

出　　处：《医学分子生物学杂志》2016年第6期341-344,共4页Journal of Medical Molecular Biology

摘　　要：目的：研究粘蛋白（ mucin， Muc）在胆囊胆固醇结石和胆囊息内胆囊组织中的表达和分泌差异。方法纳入2010年6月至2015年6月在我院行胆囊切除术患者117为研究对象，其中单纯胆囊胆固醇结石患者78例，胆囊息肉患者39例，分析和比较两组患者胆囊组织中粘蛋白mRNA的相对表达水平，以及胆汁中粘蛋白的浓度，并进一步分析两者间的相关性。结果胆固醇结石患者胆囊组织中Muc1、 Muc2、Muc5AC和Muc5B mRNA的相对表达水平均明显高于胆囊息肉患者，其差异均具有统计学意义（P＜0．05）。而胆固醇结石患者胆囊组织中Muc3 mRNA的相对表达水平明显低于胆囊息肉患者，其差异具有统计学意义（P＜0．05）。另外，胆固醇结石患者胆汁中粘蛋白浓度明显高于胆囊息肉患者，其差异具有统计学意义（P＜0．05）。结论粘蛋白参与了胆囊胆固醇结石的形成和发展，可以作为胆囊胆固醇结石的治疗靶点。Objective To examine the differences in the expression and secretion of mucin be-tween patients with cholesterol gallstone disease and with gallbladder polyps. Methods One hun-dred and seventeen patients who underwent cholecystectomy between June 2010 and June 2015 at our hospital were enrolled in the study. There were 78 cases of cholesterol gallstone disease and 39 cases of gallbladder polyps. The relative expression levels of mucins mRNA in gallbladder tissues and the levels of mucin in gallbladder bile were measured. Results The relative mRNA levels of Muc1 , Muc2 , Muc5 AC and Muc5 B were significantly higher in patients with cholesterol gallstone disease than in patients with gallbladder polyps （ P〈0. 05 ） . The relative mRNA levels of Muc3 were sig-nificantly decreased in patients with cholesterol gallstone disease when compared with those with gallbladder polyps （ P 〈0. 05 ） . The mucin level in bile was significantly higher in patients with cholesterol gallstone disease than that in patients with gallbladder polyps （ P〈0. 05 ） . Conclusion Mucins participate in the initiation and development of gallbladder cholesterol gallstone, whichcan be used as a therapeutic target for cholesterol gallstone disease.

关 键 词：粘蛋白 胆囊 胆固醇结石 

分 类 号：R657.4[医药卫生—外科学]