芒果转录因子NAC的克隆与表达模式分析  

Molecular Cloning and Expression Analysis of a NAC Transcription Factor from Mango

作　　者：余海霞[1] 罗聪[1] 徐趁 何新华[1] 

机构地区：[1]广西大学农学院广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《分子植物育种》2016年第1期38-44,共7页Molecular Plant Breeding

基　　金：广西自然科学基金(2013GXNSFDA019011);广西自然科学基金(2014GXNSFBA118102);广西高校科研项目(YB2014009)共同资助

摘　　要：NAC转录因子在植物生长发育、信号传导和逆境胁迫应答方面起重要的作用。本研究根据芒果c DNA-SCo T差异显示中发现的c DNA片段设计特异引物,通过改良RACE技术得到了芒果NAC基因的全长c DNA序列。序列分析显示,该c DNA全长为1 167 bp,开放阅读框长度为1 002 bp,编码334个氨基酸,推测其分子量为37.41 k D,等电点为8.76,属于NAM基因家族,命名为Mi NAC。利用实时荧光定量技术对Mi NAC基因在芒果4℃低温胁迫、盐胁迫和PEG-6000胁迫后0、24 h、48 h和72 h时叶片和茎中表达模式进行分析,实验结果显示,这三种逆境处理均诱导芒果Mi NAC基因的表达,其中Mi NAC基因对4℃低温胁迫响应程度最高,其次是PEG胁迫和盐胁迫。结果表明,该基因参与了芒果逆境胁迫的分子调控。The NAC transcription factors play important roles in plant development, signal transduction and response to various kinds of stresses. Based on the c DNA fragment from c DNA-SCo T different expression analysis, specific primers were designed to clone a full-length sequence of NAC gene by using modified RACE techniques from mango. Bioinformatics analysis showed that the full length of Mi NAC gene was 1 167 bp,containing a 1 002 bp complete open reading frame, which encoded a polypeptide of 334 amino acids with a predicted molecular mass of 37.41 k D and theoretical isoelectric points(p I) of 8.76, belonging to the NAM gene family and named Mi NAC gene. The expression of Mi NAC gene under the stress treatments of low temperature,Na Cl and PEG-6000 at 0, 24 h, 48 h and 72 h were detected by quantitative real-time fluorescent PCR. The expression results indicated that Mi NAC gene were regulated by the three kinds of stress treatments. The Mi NAC gene was significantly up-regulated by 4℃ low temperature treatment, as well as by the drought and salt treatment.It suggested that Mi NAC gene play a role in stress tolerance in mango.

关 键 词：芒果 NAC基因 胁迫 表达模式 

分 类 号：S667.7[农业科学—果树学]