甜菜Owen型质核互作雄性不育系及保持系花蕾cDNA-AFLP多态性分析  

Bud of Sugar Beet c DNA-AFLP Polymorphism Analysis of Owen-type Male Sterile Line and Maintainer Line

作　　者：孟祥雯 程大友[1] 崔杰[1] 罗成飞[1] 代翠红[1] 刘天骄[1] 史淑芝[1] 刘一珺 

机构地区：[1]哈尔滨工业大学,哈尔滨150090 [2]湖北科技学院,咸宁437100

出　　处：《分子植物育种》2016年第1期135-140,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(31271781;31371685)资助

摘　　要：本研究以Owen型胞质雄性不育甜菜的不育系及保持系花蕾为实验材料,通过c DNA-AFLP分子标记方法,从转录组水平对不育系及保持系的甜菜花蕾进行基因多态性分析,通过128对AFLP引物筛选,在保持系和不育系中共找到并且成功回收到60条特异性差异表达条带,其中保持系35条,不育系25条。在成功回收测序的30条差异片段中,18条NCBI-Blast成功检索到对应的同源已知功能基因,分别为:编码假定蛋白、编码转运蛋白G家族成员、编码液泡分选受体、编码细胞色素c1、编码半乳糖醛酸转移酶、编码转录因子、编码DNA指导的RNA聚合酶、编码泛素结合酶、编码蔗糖合成酶、编码丝/苏氨酸蛋白激酶的基因,经筛选、验证后得到4个阳性差异片段。这些片段可作为甜菜Owen型不育系及保持系快速鉴定的依据,对缩短甜菜育种年限,加快育种进程具有重要意义。In this research, Owen-type cytoplasmic male sterile lines and the maintainer line beet buds were used as experimental material to analyze the buds of beet gene polymorphism on transcription level. By using 128 pairs of primers screening, we found and recovered 60 specific different bands in maintainer line and sterile line, with 35 bands in maintainer line, 25 bands in sterile line. Among 30 recovery bands, 18 bands obtained corresponding homologous known function genes through NCBI-Blast, which were encoding hypothetical protein, transporter G family member, vacuolar-sorting receptor, cytochrome c1, galacturonosyltransferase, transcription factor, DNA-directed RNA polymerase, ubiquitin-conjugating enzyme, sucrose synthetase, ser/thr protein kinase gene, respectively.After screening and verifying, we obtained four positive different bands. These fragments could be used as evidence of fast identify on sterile line or maintainer line of Owen-type sugar beet, as well as can shorten the breeding period and promote the breeding process.

关 键 词：甜菜 Owen型胞质雄性不育 花蕾 CDNA-AFLP 

分 类 号：S566.3[农业科学—作物学]