蚜虫取食诱导高粱多酚氧化酶(PPO)基因的表达及酶活性分析  

Polyphenol Oxidase(PPO) Gene Expression and Enzyme Activity Analysis in Sorghum Induced by Aphids Feeding

作　　者：邵玉涛[1] 王振杰[1] 郭江波[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,包头014010

出　　处：《分子植物育种》2016年第2期280-285,共6页Molecular Plant Breeding

基　　金：内蒙古自治区高等学校科学研究项目(NJZY152);内蒙古科技大学创新基金项目(2014QDL008)共同资助

摘　　要：多酚氧化酶(PPO)在植物防御病虫害中发挥了重要作用。为了探明PPO在高粱对高粱蚜的防御反应中所发挥的作用,本研究利用抗蚜品种HN16和感蚜品种BTx623两种材料,对高粱中8个PPO基因接虫3 d和5 d的表达模式进行了分析,并对同一时期的PPO活性进行测定。结果表明,在蚜虫取食过程中,在感蚜品种BTx623中,随着蚜虫取食时间的延长,除PPO3外,其他7个PPO基因的表达量均呈现先上升,后降低的趋势;而在抗蚜品种HN16中,8个PPO基因的表达量持续上升,PPO的酶活也有所升高,可见,PPO在HN16的抗虫反应中发挥着一定的主动防御功能,在这个过程中,3个成员PPO1,PPO5和PPO6可能起了主要作用。Polyphenol oxidase(PPO) plays an important role in plant defense against insect pests. In order to find out the role of PPO in the defense response of sorghum to sorghum aphid, the expression patterns of 8 PPO genes in sorghum were analyzed by using HN16(resistant to aphid) and BTx623(susceptible to aphid) two materials. The results showed that except PPO3 the expression levels of other 7 PPO genes increased at first and then decreased in BTx623 during aphid feeding. While the expression levels of 8 PPO genes increased sustainly during aphid feeding in HN16 and the PPO enzyme activity increased too. Obviously, PPO played a certain active defense function against aphids in HN16, and in the process PPO1, PPO5 and PPO6 may have played a major role.

关 键 词：高粱 多酚氧化酶 基因表达 酶活分析 

分 类 号：S435.14[农业科学—农业昆虫与害虫防治]