海陆渐渗系棉花F_2群体SSR标记偏分离的遗传分析  

Genetic Analysis on Segregation Distortion of Molecular Markers in F_2 Population of Introgression Lines

作　　者：戎福喜 魏亦农[1] 李志博[1] 李衡[1] 汤丽魁[1] 

机构地区：[1]石河子大学农学院新疆兵团绿洲生态农业重点实验室,石河子832003

出　　处：《分子植物育种》2016年第2期424-430,共7页Molecular Plant Breeding

基　　金：国家重点基础研究发展计划(2012CB722205);国家自然科学基金(31360265);石河子大学动植物育种专项(gxjs2013-y203);石河子大学高层次人才科研启动项目(RCZX201313)共同资助

摘　　要：本研究以海陆渐渗系13-1与陆地棉辽棉12构建的F2群体为材料,利用4 500对中具有多态性的84对SSR引物进行群体间分析。研究结果显示:在p<0.05显著水平上,共有19个分子标记表现偏分离,占总标记数的25.3%,其中有8个标记偏向父本辽棉12,占偏分离标记总数的42.1%;10个标记偏向母本13-1,占偏分离标记总数的52.63%;1个标记偏向杂合体,占偏分离标记总数的5.27%。这些标记在图谱上有两种分布形式,分别为成簇分布和孤立分布。在7条不同的染色体上均发现偏分离标记,其中在1染色体上发现1个热点区域。本研究分析了产生偏分离的原因,并讨论偏分离标记对QTL定位的影响。F-_2 materials derived from a cross between introgression lines 13-1×Liaomian 12 were used as mapping population in cotton. Among the 84 polymorphic markers analyzed, 19 markers(25.3%) showed the genetic distortion(p<0.05) in the F2 population. Among these segregation distortion SSR markers, 8 SSR marker alleles were distorted to the male parent Liao12(42.1%), 10 SSR markers allels were distorted to the female parent 13-1(52.61%), and one SSR marker alleles were distorted to the heterozygote(5.27%). These segregation distortion SSR markers were in cluster of tightly loci or single marker, which distributed in 7 different chromosomes with hot region in one of them. In this research, reasons for segregation distortion and effects of segregation distortion on genetic mapping and QTL analysis were discussed.

关 键 词：棉花 海陆渐渗系 SSR分子标记 偏分离 遗传分析 

分 类 号：S562[农业科学—作物学]