切花非洲菊组织培养及快速繁殖研究  

Study on Tissue Culture and Rapid Propagation of Cut Gerbera jamesonii Bolu

作　　者：成晟[1] 罗福来[1] 钟定业 李智[2] 

机构地区：[1]海南大学园艺园林学院,海口570282 [2]中国热带农业科学院热带作物品种资源研究所,儋州571737

出　　处：《分子植物育种》2016年第3期693-698,共6页Molecular Plant Breeding

基　　金：国家科技支撑项目(2009BADA1B03);海南省教育厅科学研究项目(HNKY2014-21)共同资助

摘　　要：本试验以花托为外植体对适宜在海南省栽培的耐高温切花非洲菊品系进行了组织培养,成功获得再生植株,并建立了快速繁殖体系。试验将外植体材料,经消毒后,在无菌条件下,分别接种到不同成分配比的固体培养基上(包括愈伤组织及芽诱导培养基,丛生芽增殖培养基,生根培养基)进行培养。通过比较、分析等方法,筛选出最适宜的培养基。并通过实际操作获得了炼苗、移栽等相关技术。试验结果表明:非洲菊组织培养的最佳诱导培养基是MS+BA 6.0 mg/L+IAA 0.3 mg/L+蔗糖30 g/L+卡拉胶7 g/L;最佳增殖培养基是MS+BA 0.5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+卡拉胶7 g/L;最佳生根培养基是1/2MS+IBA 0.5 mg/L+NAA0.05 mg/L+蔗糖15 g/L+卡拉胶7 g/L。并且本试验在炼苗、移栽等相关技术方面也得出了一定的结论。为非洲菊优良切花品种在海南省快速推广和新品种研发等提供技术支持。The reborned plants were successfully acquired by taking the receptacle as explant in the tissue culture of Gerbera jamesonii.The variety used in the experiment is adaptive to the high temperature in Hainan Province,and the system of rapid propagation was also established.In the experiment,the explants were inoculated onto different solid medium(medium for callus and shoot induction,proliferation medium for multiple shoot and rooting medium) after sterilizing.It was available to select the best medium by comparison and analysis.At the same time,relative techniques about acclimatization and transplanting of Gerbera jamesonii seedling were explored during actual operation.The results of experiment indicated that the superior induction medium is MS +BA 6.0 mg/L +IAA 0.3 mg/L+Sucrose 30 g/L+Carrageenan 7 g/L,the best proliferation medium is MS +BA 0.5 mg/L+NAA0.1 mg/L+Sucrose 30 g/L+Carrageenan 7 g/L,the best rooting medium is 1/2MS + IBA 0.5 mg/L+NAA 0.05 mg/L+Sucrose 15 g/L +Carrageenan 7 g/L.And we got some useful results about acclimatization and transplanting are acquired so as to offer technical support for the R&D of new variety and rapid promotion of superior cut flower varieties of Gerbera jamesonii in Hainan.

关 键 词：切花 非洲菊 组织培养 快速繁殖 花卉 

分 类 号：S567.239[农业科学—中草药栽培]