木薯蔗糖转运蛋白(SUT)家族基因的染色体物理定位  

The Chromosome Physical Location of the SUT Family Genes in Cassave

作　　者：李晶晶[1,2] 王英[1] 高和琼[1] 李开绵[2] 庄南生[1] 

机构地区：[1]海南大学农学院热带作物种质资源保护与开发利用教育部重点实验室,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,儋州571737

出　　处：《分子植物育种》2016年第4期794-802,共9页Molecular Plant Breeding

基　　金：国家973计划项目(2010CB126606);海南省重大科技专项(ZDZX2013023);"中央财政支持中西部高校提升综合实力专项资金"共同资助

摘　　要：本研究以木薯华南6号(SC6)根尖为材料,制备染色体标本。利用原位PCR技术和荧光原位杂交技术,结合核型分析,对木薯4个蔗糖转运蛋白(sucrose transporter protein,SUT)基因在染色体上的具体位置进行了定位分析。结果表明:SUT1位于第14号染色体的长臂上,SUT4.2位于第15号染色体的长臂上,SUT2位于第17号染色体的短臂上,SUT4.1位于第7号染色体的短臂上;信号检出率依次对应为:14.1%、17.1%、12.3%和13.3%;信号位点到着丝粒的百分距离依次对应:42.80±0.1、23.14±0.5、44.89±0.5及37.38±0.1。这4个基因位于不同的染色体上,互为独立基因。本研究还对这些基因与其他已定位基因的位置关系进行了讨论。这些可以为木薯分子标记辅助育种和基因组选择育种体系提供分子细胞遗传学依据。In this study, chromosome specimens were prepared with root tip cells of cassava SC6. IS-PCR(in situ PCR) and FISH(fluorescence in situ hybridization) were used to physically locate the 4 SUT genes of Manihot esculenta Crantz. The results indicated that: the genes of SUT1, SUT4.2, SUT2 and SUT4.1 were respectively located on the long arms of the chromosome 14 and 15, on the short arm of the chromosome 17 and 7 in Cassava SC6; the signal detection rates were 14.1%, 17.1%, 12.3% and 13.3%, respectively; the percentage distances from the centromere to signal site were 42.80 ±0.1, 23.14 ±0.5, 44.89 ±0.5 and 37.38 ±0.1, respectively. The linkage analysis was proceeded with the located genes, then we found SUT1, SUT2, SUT4.1 and SUT4.2 genes were separate genes since they distributed on different chromosomes of Cassava. The physical location of those genes will provide molecular genetics basis for cassava molecular marker assisted breeding and genomes selective breeding system.

关 键 词：木薯SC6 SUT基因 原位PCR 荧光原位杂交 物理定位 

分 类 号：S533[农业科学—作物学]