三色堇VwMYB8基因的克隆及其在月季中的瞬时表达分析  

Cloning of VwMYB8 Gene from viola×wittrikianan Gams. and Its Transient Expression in Rose

作　　者：曾媛[1] 龚胜[1] 李婧[1] 孙海燕[1] 王健[1] 杨文汉[1] 李霆格 赵安瑾 梅晶晶[1] 

机构地区：[1]海南大学园艺园林学院热带作物种质资源保护与开发利用教育部重点实验室,海口570228

出　　处：《分子植物育种》2016年第4期844-850,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31060265);海南大学中西部计划学科建设项目(ZXBJH-XK008)共同资助

摘　　要：三色堇花色艳丽,品种丰富,是重要的冬春季花卉。本研究采用黄底紫斑品种‘梦蝶’为研究材料,克隆花瓣中可能参与花青素累积起调控作用的MYB基因VwMYB8,结果显示:克隆得到的cDNA全长为1 078 bp,经ORF Finder分析发现其有长度为849 bp的开放阅读框,编码282个氨基酸;构建瞬时表达载体pXCS-VwMYB8,采用粒子轰击的方法作用于月季‘粉扇’花瓣细胞,经过48 h培养后观察转化材料细胞中色素的变化,转化的细胞颜色明显加深,表明基因VwMYB8可以提高月季花瓣细胞中色素的含量。Viola ×wittriki anan is an important flower in winter and spring for its beautiful flower colors and rich cultivars. In this study the yellow cultivar‘Mengdie'with purple blotch on petals was used as research material to clone VwMYB8 of MYB gene in the petal which may be involved in regulation of anthocyanin accumulation. The results showed that the c DNA full length of this gene was 1 078 bp. ORF Finder analyses showed that the open reading frame was 849 bp, encoding 282 amino acids. A transient expressed vector p XCS-VwMYB8 was constructed, using particle bombardment assay to transfer this gene into the petal cell of rose‘Pink fan'. The distinct pigmentation was observed in the transformed rose petal cells after 48 h incubation. The result of transient expression indicated that VwMYB8 gene may improve pigment accumulation in cells of rose petals.

关 键 词：三色堇 VwMYB8 瞬时表达 花青素调控 

分 类 号：S68[农业科学—观赏园艺]