木榄超活性突变基因BgSOS1-3000的分离及功能分析  

Isolation and Function Analysis of a Super-active Mutant Gene of BgSOS1-3000 from Bruguiera gymnorhiza

作　　者：郭晓颖[1] 范亚飞[1] 周扬[1] 杨莹[1] 江行玉[1] 

机构地区：[1]海南大学农学院海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《分子植物育种》2016年第4期851-857,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31260218;31160185)资助

摘　　要：采用PCR的方法,分离到木榄质膜Na^+/H^+逆转运蛋白基因BgSOS1 3'-末端缺失的突变基因BgSOS1-3000。BgSOS1-3000缺少了SOS1蛋白末端的自抑制区。构建酵母表达载体BgSOS1-pYPGE15和BgSOS1-3000-p YPGE15,在酵母突变体AXT3K中分析它们的功能,结果发现与转全长BgSOS1基因的酵母相比,转突变基因BgSOS1-3000的转基因酵母耐盐性更强,可在200 mmol/L Na Cl条件下生长,而且超活性突变体增强耐盐性不依赖于植物体内的SOS2/SOS3蛋白复合体。通过测定转基因酵母的离子含量,发现转BgSOS1-3000基因的酵母中的Na+显著低于转BgSOS1基因的酵母,说明BgSOS1-3000超活性突变体通过外排更多的Na+能提高酵母的耐盐性。这为提高植物耐盐性提供了新材料。In this study, the authors isolated a 3'-truncated gene BgSOS1-3000 of BgSOS1, a mutant deleted an auto-inhibitory domain at the C-terminus of BgSOS1, from Bruguiera gymnorhiza by PCR method. The plasmid BgSOS1-p YPGE15 and BgSOS1-3000-p YPGE15 were constructed and transformed into the yeast mutant strain AXT3 K. Compared with the BgSOS1 wild-type gene, the yeast cells transformed with BgSOS1-3000 gene showed a higher salt tolerance and could grow well under 200 mmol/L Na Cl. In addition, the super active mutant had higher salt tolerance independent from regulating by the SOS2/SOS3 complex. The sodium content in the yeast cells expressing BgSOS1-3000 gene was significantly lower than that expressing BgSOS1 gene. These results indicated that the yeast cells transformed with the super active gene BgSOS1-3000 could exclude more Na+ and conferred salt tolerance, which gave a new material for increasing plant salt tolerance.

关 键 词：细胞膜 Na+/H+逆转运蛋白 木榄 超活性突变体 

分 类 号：Q943.2[生物学—植物学]