水稻细胞自发荧光加强对抵抗稻瘟病菌侵染的作用  

The Contribution of Autofluorescence Enhancement in Rice Resistance to Magnaporthe oryzae

作　　者：梁艳丽[1] 赵婧[1] 刘林[1] 杨静[1] 李成云[1] 

机构地区：[1]云南农业大学农业生物多样性与病害控制教育部重点实验室,昆明650201

出　　处：《分子植物育种》2016年第6期1568-1573,共6页Molecular Plant Breeding

基　　金：973计划前期研究专项(2012CB722900);国家自然科学基金(81460583)共同资助

摘　　要：稻瘟病是为害水稻生产最为严重的病害之一。弄清水稻与稻瘟病菌的互作机制对于稻瘟病的防控至关重要。本研究通过对不同抗性品种(YJ19,IRBL22和LTH)不同侵染阶段的细胞观察,发现水稻品种YJ19对稻瘟病菌16T抗性最强,其次为IRBL22、LTH抗性最弱;抗性品种自发荧光增强强度显著大于感病品种。且抗性越强,其荧光增强点的密度越大,荧光增强频率越高,侵染点荧光增强细胞数目越多,荧光点越大。在侵染后期感性品种细胞产生显著的褐化现象,而抗性品种褐化现象不明显。水稻细胞对稻瘟病菌侵染出现明显细胞形态变化的关键时间点为接种后72 h。研究结果可为培育广谱、持久抗性水稻品种,制定抵抗稻瘟病害的新措施提供一定的参考价值,具有重要的现实意义。Rice blast disease is one of the most devastating of all rice diseases. To make clear the mechanisms of rice and Magnaporthe oryzae interaction is very important in rice production. Three rice varieties(YJ19, IRBL22 and LTH) were used in this research as experimental materials to study the rice cell response to the infection of M.oryzae at the different infection stages. The results showed that YJ19 had the highest resistance to blast fungus 16 T followed by IRBL22, LTH had the lowest resistance. The autofluorescent enhancement degree of incompatible cultivars was much more than that of the compatible cultivar. Furthermore, the cultivars with higher resistance had higher density, frequencyand bigger spots of autofluorescence enhancement. Cells of compatible cultivar browned generally at the later stage of infection comparied with the cells of incompatible cultivars with no browned cells.The key time point for morphological changes of rice cells was 72 h after inoculation. The observation might provide important information for prevalence and valuable breeding and disease control strategies.

关 键 词：稻瘟病 水稻品种 自发荧光 抗性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]