单基因型匍匐翦股颖茎段的愈伤组织诱导和植株再生  

Callus Induction and Plantlet Regeneration of Single Genotype Creeping Bentgrass Stem

作　　者：刘海珍[1] 施杨[1] 梁中银[1] 支俊凯 徐吉臣[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《分子植物育种》2016年第6期1582-1586,共5页Molecular Plant Breeding

基　　金：国家自然科学基金项目(J1103516;31128016);北京市自然科学基金项目(5112015)共同资助

摘　　要：为建立匍匐翦股颖愈伤组织再生体系,通过种子愈伤组织诱导和再生实验,筛选出克隆系Penn A4-1。进一步以克隆系的茎段为材料,建立了高效稳定的单一基因型Penn A4-1愈伤组织诱导和再生体系。愈伤组织诱导和愈伤组织增殖的最适培养基均为MS+1 mg/L 2,4-D,出愈率和增殖率分别达到91.67%和169.25%;愈伤组织分化的最适培养基为MS+2 mg/L 6-BA+0.1 mg/L NAA,分化率达到52.38%。高效愈伤组织再生体系的建立,为匍匐翦股颖品种Penn A4持续稳定的基因工程研究奠定了重要的基础。For the establishment of callus regeneration system in creeping bentgrass, the research here screened out a clone line Penn A4-1 based on seeds callus induction and regeneration test. Further, a high efficient and stable system of callus induction and regeneration from single genotype of the clone line Penn A4-1 stem was established.The proper medium for callus induction and proliferation both was MS+1 mg/L 2,4-D with the rate of 91.67% and169.25%, respectively. The proper medium for callus differentiation was MS +2 mg/L 6-BA+0.1 mg/L NAA with the rate up to 51.43%. The establishment of high efficient callus regeneration system would lay an important foundation for the sustained and stable gene engineering study of creeping bentgrass species Penn A4.

关 键 词：匍匐翦股颖 基因型 茎段 愈伤组织 再生 

分 类 号：S688.4[农业科学—观赏园艺]