响应曲面法优化朱砂根中岩白菜素的提取工艺  

Optimization of extracting process of bergenin from the roots of Ardisia crenata by response surface methodology

作　　者：胡文杰[1] 司志国[2] 许樟润 李宁[2] 李冠喜 HU Wen-jie SI Zhi-guo XU Zhang-run LI Ning LI Guan-xi(College of Life Sciences, Jinggangshan University, Ji ＇an 343009, China Henan Vocational and Technical College, Environmental Art Engineering, Zhengzhou 450046, China Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China)

机构地区：[1]井冈山大学生命科学学院,吉安343009 [2]河南职业技术学院环境艺术工程系,郑州450046 [3]连云港农业科学院,连云港222006

出　　处：《药物分析杂志》2016年第12期2125-2130,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：江苏省自然科学基金(BK20151278);江苏省江苏苏北科技项目(BN2014029)

摘　　要：目的:采用响应曲面法优化朱砂根中岩白菜素的最佳工艺条件。方法:在单因素的基础上运用Box-Behnken中心组合设计以提取时间、液固比和提取功率3因素3水平的试验模型,建立回归方程。采用响应曲面法对试验结果进行了分析,对朱砂根中岩白菜素的提取工艺条件进行优化。结果:模拟得到的最佳提取条件:提取时间为41.21 min,液固比为10.14∶1(m L·g^(-1)),提取功率为108.83 W,此时的最大提取率为2.233%。为了便于实际操作,修正条件为:提取时间42 min,液固比为10∶1(m L·g^(-1)),提取功率为109 W,验证试验结果平均提取率为2.318%,与模型预测值较为接近。结论:本文所建立的数学模型精度高(P<0.01),可对朱砂根中岩白菜素超声波提取工艺进行分析和预测。Objective： To optimize the technology for bergenin extraction from the roots of Ardisia crenata. Methods： Based on the single factor test, the three factors of extracting time, solid to liquid ratio, and extracting power were studied by the orthogonal test developed from Box-Behnken central composite test design. Meanwhile, the relative equation was established. Using the response surface method, the result was analyzed and the extracting process was optimized. Results： The optimized conditions were as follows：the extracting time was 41.21 min, solid to liquid ratio was 10.14：1 （mL·g-1）, the extrating power was 108.83 W, and the extraction rate was 2.233%. In order to facilitate the practice, the conditions were modified as follows：the extracting time was 42 min, solid to liquid ratio was 10：1 （mL·g-1）, and the extracting power was 109 W. The experimental results was verified with an average extraction rate of 2.318%, which was close to the model predicted value. Conclusion： The mathematical model established has high presision （P〈0.01）, which can be used to analyze and predict the extraction process of bergenin from Ardisia crenata.

关 键 词：药用植物 富贵籽 铁凉伞 朱砂根 岩白菜素 响应曲面法 提取工艺 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]