一测多评法测定艾叶中6个有机酸类成分的含量  

Simultaneous determination of six organic acids in Artemisiae Argyi Folium by QAMS

作　　者：于晓[1,2] 戴衍朋[3] 周倩[3] 孙立立[3] YU Xiao DAI Yan-peng ZHOU Qian SUN Li-li(Shandong University of Traditional Chinese Medicine, Jinan 250355, China Shandong Academy of Chinese Medicine, Jinan 250014, China Yantaishan Hospital, Yantai 264001, China)

机构地区：[1]山东中医药大学,济南250355 [2]烟台山医院,烟台264001 [3]山东省中医药研究院,济南250014

出　　处：《药物分析杂志》2016年第12期2131-2138,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然基金项目-艾叶炭炮制原理研究(81173545);2008年中医药行业科研专项(200807039)

摘　　要：目的:建立同时测定艾叶中6个有机酸类成分含量的一测多评法。方法:采用Thermo Syncronis C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1%甲酸水溶液-乙腈,梯度洗脱,流速0.8 m L·min^(-1),检测波长325 nm,柱温30℃。以绿原酸为内参物,分别计算与新绿原酸、隐绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的相对校正因子,实现一测多评。同时利用外标法和一测多评法测定6个有机酸类成分的含量。比较2种方法的测定结果是否存在差异。结果:一测多评法与外标法测定结果无显著差异。实验所测得的相对校正因子重复性良好。6批不同产地艾叶样品中新绿原酸、绿原酸、隐绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的含量范围分别为0.15~0.58、0.50~2.65、0.27~0.89、0.62~4.29、1.18~10.72、0.99~6.69 mg·g^(-1)。结论:一测多评法可以用于艾叶中6个有机酸类成分的含量测定,且具有可行性与经济性。Objective： To establish a quality evaluation method to simultaneously determine the contents of six organic acids in Artemisiae Argyi Folium with a single-marker （QAMS）.Methods： An HPLC method was established with a Thermo Syncronis C18 （4.6 mm×250 mm, 5 μm） with gradient elution using 0.1% formic acid solution-acetonitrile as the mobile phase.The flow rate was 0.8 mL·min-1, the detection wavelength was 325 nm, and the column temperature was 30℃.Chlorogenic acid was used as the internal reference substance.The relative correlation favtors （RCF） of neochlorogenic acid, cryptochlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid to chlorogenic acid were calculated.The content of these six organic acids were determined by the external standard method and QAMS method respectively.The QAMS method was validated through comparison of the results obtained by the two different methods.Results： No significant difference was found between the two methods.The RCF showed good reproducibility.The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid in 6 batthes of samples of Artemisiae Argyi Folium were 0.15-0.58 mg·g-1, 0.50-2.65 mg·g-1, 0.27-0.89 mg·g-1, 0.62-4.29 mg·g-1, 1.18-10.72 mg·g-1 and 0.99-6.69 mg·g-1, respectively.Conclusion： The QAMS method showed favorable feasibility and economy, which can be used to determine the content of six organic acids in Artemisiae Argyi Folium.

关 键 词：艾叶 有机酸类成分 绿原酸 新绿原酸 隐绿原酸 二咖啡酰奎宁酸 一测多评 相对校正因子 

分 类 号：R917[医药卫生—药物分析学]