宠物犬布氏杆菌的PCR检测及部分序列分析  

PCR Detection and Partial Sequencing Analysis of Brucella canis from Pet Dogs

作　　者：孙明[1] 刘巧荣[1] 宋文静 乔明明[1] 顾强 张谦[1] 刘伯华[1] 杨雪松 陈西钊[1] 

机构地区：[1]北京世纪元亨动物防疫技术有限公司,北京100094 [2]北京关忠动物医院,北京100025

出　　处：《畜牧兽医学报》2016年第12期2520-2525,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十三五"国家重点研发计划(2016YFD0501006)

摘　　要：对北京关忠动物医院送检的2例疑似感染布氏杆菌宠物犬的7份分泌物进行了布氏杆菌普通PCR、快速实时荧光PCR检测和Multi-PCR鉴定,并对部分基因序列测序分析,旨在对宠物犬感染犬布氏杆菌作出确诊,为宠物犬感染布氏杆菌诊断与防控提供资料。结果显示,布氏杆菌快速实时荧光检测试剂盒对两例犬的7份分泌物检测结果均为布氏杆菌阳性;Multi-PCR检测结果显示,感染犬布氏杆菌属于犬种布氏杆菌,且为非典型犬布氏杆菌;序列分析进一步验证了患犬感染样品扩增的序列均为布氏杆菌序列：BSCP31表面蛋白序列与已经发布的序列相比相似性在99%~100%;OMP25序列与中国内蒙古分离的犬布氏杆菌xue1分离株和韩国Brucella canis HSK A52141分离株亲缘关系最为亲近,核苷酸相似性均为99.9%;16SrRNA序列显示,进口斗牛犬和泰迪犬感染菌株均属于布氏杆菌序列。以上试验结果表明两例宠物犬均为犬布氏杆菌感染,这些数据将为布氏杆菌病的筛查和防控提供一定的基础。Two cases of pet dogs were confirmed to be infected with Brucella canis based on detection to their excretion by use of the BRU real time PCR and conventional PCR kits,and the partial genetic sequences of the Brucella canis infected were characterized.The results showed that the Brucellastrains from the pet dogs belong to the Brucella canis,and atypical canine brucellosis.Phylogenetic analysis of BSCP31 gene sequences showed 99%-100% nucleotide identity with the published sequences in GenBank,and OMP25 gene showed 99.9% nucleotide identity between Brucella canis xue1 strain and Brucella canis HSK A52141 strain.Sequence analysis on16 SrRNA showed the strains from French Bulldog and Teddy Dog belong to Brucella.These data might provide a valuble infomation for future screening and prevention of canine brucellosis.

关 键 词：布氏杆菌 宠物犬 PCR检测 序列分析 

分 类 号：S852.612[农业科学—基础兽医学]