辅料因素对炮制蒙药诃子制草乌质量的影响  

Influence of Fructus Chebulae water solution on quality of Mongolian medicine Aconiti Kusnezoffii Radix Praeparata

作　　者：刘帅[1] 李卫飞[1] 李妍[1] 许金凯[1] 刘晓艳[1] 李飞[1] 杜红[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2016年第11期949-954,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81102815);北京市自然科学基金项目(No.7162120);北京中医药大学研究生自主课题(No.2016-JYB-XS069)

摘　　要：目的探讨辅料因素对蒙药诃子制草乌质量的影响,阐明辅料发挥炮制减毒作用的原理,优选出最佳炮制工艺。方法煎煮制备不同比例的诃子汤作为炮制辅料,分别测定其p H值、总鞣质含量。并用不同比例的诃子汤分别炮制草乌,采用反相高效液相色谱法检测各炮制品中代表毒效成分的6种生物碱含量。结果随诃子用量增加,诃子汤中总鞣质含量不断升高;诃子汤p H值先减小,后基本维持稳定;诃子制草乌中6种生物碱总量先逐渐升高后变化不规律。其中草乌、诃子比例为2∶1时,诃子制草乌中生物碱含量达到最低,该比例与1986年《内蒙古蒙药材标准》中对诃子、草乌炮制用量的比例一致。结论诃子用量及炮制辅料是否新鲜均会影响诃子制草乌中生物碱含量大小,《内蒙古蒙药材标准》对诃子、草乌炮制用量比2∶1具有一定的科学性,建议继续沿用。Objective To study the influence of different concentration of Fructus Chebulae（ Hezi） water solution on the quality of Aconiti Kusnezoffii Radix Praeparata（ Zhicaowu）,so as to optimize the processing technology and to find out the principle of detoxification of Hezi in processing. Methods Taking Hezi water solution with different concentration as the excipients,p H and content of total tannins were determined. Then content of six alkaloids,indicator of toxic effect,in Zhicaowu were measured by reversedphase HPLC. Results With the increasing of the concentration of Hezi water solution,the content of total tannins increased,p H decreased at first,then kept stable,and the total six alkaloids increased slowly followed by irregularity. When the content ratio of Caowu to Hezi was 2 ∶ 1,consistent with Standard of Inner Mongolia Mongolian Medicines,the total alkaloids content was least. Conclusion Concentration of Hezi water solution and storage time changed the content of alkaloids in Zhicaowu. The content ratio of Hezi to Caowu recorded in Standard of Inner Mongolia Mongolian Medicines（ in 1986） was scientific and valid.

关 键 词：诃子制草乌 炮制 辅料 生物碱含量 P H值 鞣质 

分 类 号：R283.1[医药卫生—中药学]